Exploration on the core prescription mining and mechanism of treating malignant tumors from Jueyin theory
Objective To explore the core prescription of Professor Li Zhong's treatment of malignant tumors based on Jueyin theory and carry out in experimental verification of the inhibitory effect of drug-containing serum on tumor cells.Methods Professor Li Zhong's prescription data of patients with tumors from January 2022 to June 2023 in the Department of International,Dongzhimen Hospital,Beijing University of Chinese Medicine were collected.Through the ancient and Modern Medical Case Cloud Platform V 2.3.7,the drug frequency,natures,flavors,and channel tropism were analyzed.The cluster analysis,correlation analysis,complex network analysis and the core prescription were carried out.Eight SPF 6-8 week old SD male rats were divided into Chinese medicine group and control group by random number table method,with four rats in each group.The Chinese medicine group and control group were gavaged with 2 ml of core prescription herbal decoction and the same dose of saline for 7 d,respectively,and the drug-containing serum was extracted.CCK8 method was used to determine the optimal intervention concentration and intervention time of drug-containing serum on A549 and HepG2 cells.Blank groups were set up respectively:complete medium culture,no drug;Chinese medicine group:intervention with the best concentration of drug-containing serum;control group:intervention with the same dose of blank serum.Transwell assay was used to detect cell migration and invasion ability.Flow cytometry was used to detect apoptosis and cell cycle.Western blot was used to detect the expression of apoptosis and epithelial mesenchymal transition related proteins.Results A total of 8 166 prescriptions were included,with a total of 300 kinds of drugs.The medicinal properties were warm and sweet,and the liver and lung meridians were the main meridians.The frequency of use of 16 drugs such as Radix Astragali,Carapax Trionycis,Mume Fructus,and cornus were＞90％.Combining the results of cluster analysis and complex network analysis with the experience of Professor Li Zhong,one core prescription of the pingtong yin-yang method was derived,including raw Radix Astragali,roasted Radix Astragali,Carapax Trionycis shell,Agrimoniae Herba,Mume Fructus,Codonopsis Radix,Angelicae Sinensis Radix,Rehmanniae Radix Praeparata,Lonicerae Japonicae Caulis,Paeoniae Radix Alba,Dioscoreae Nipponicae Rhizoma Fritillariae Thunbergii Bulbus,Bombyx Batryticatus,raw Concha Ostreae,and cornus.It was found that the optimal intervention concentration of the herbal medicine containing serum was 20％and the duration of the intervention was 48 h.The migration and invasion ability of A549 and HepG2 cells in the Chinese medicine group was lower than that of the control group,the apoptosis rate was higher than that of the control group,the blocked cell cycle was longer than that of the control group,and the expression levels of Bcl-2,N-Cadherin,and Vimentin were lower than those of the control group,and the expression levels of Cleaved-Caspase-3 and E-Cadherin were higher than those of the control group(P＜0.05).Conclusion By using data mining,the core prescription can be obtained.The prescription drug-containing serum can inhibit the proliferation,migration,and invasion of tumor cells.The mechanism may be related to promoting apoptosis,blocking cell cycle,and inhibiting epithelial-mesenchymal transition process.
JueyinMalignant cancerCore prescriptionYin and yang theoryData miningMechanism
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