Effect of sophoridine on the growth and invasion of prostate cancer PC-3 cells via down-regulation of β-Catenin
Objective To investigate the effect of sophoridine on the proliferation and invasion of prostate cancer PC3 cells via down-regulation of β-Catenin.Methods PC3 cells of human prostate cancer were selected,the change of proliferation capacity was detected by CCK8 assay(10,20,40,80,160,320,640 mg/L sophoridine treatment),and IC50 was calculated,and 3 cells treated with appropriate concentration were set into 50,100 and 200 mg/L groups,and no drug treatment was used as the control group.Cell scratch assay was used to detect the migration ability of each group,Transwell assay was used to detect the invasion ability of each group.The levels of key proteins in Wnt/β-Catenin pathway,such as β-Catenin,Cyclin D1 and MMP-9,were detected by Western blotting.The interaction between sophoridine and β-Catenin was predicted by molecular docking.Results CCK8 experiment showed that the inhibition rate of PC3 cells treated with 10,20,40,80,160,320,640 mg/L sophoridine was increased compared with the control group(P＜0.01).Compared with the control group,the migration and invasion ability of PC3 cells in 50,100 and 200 mg/L groups were decreased(P＜0.05),and the proteins ofβ-Catenin,CyclinD1 and MMP9 in 100 and 200 mg/L groups were down-regulated(P＜0.05).Compared with 50 mg/L group,migration ability,invasion ability,β-Catenin and CyclinD1 protein levels of PC3 cells in 100 and 200 mg/L groups were decreased(P＜0.05),and MMP9 protein levels in 200 mg/L group were increased(P＜0.05).Compared with 100 group,migration ability,invasion ability,β-Catenin and MMP9 protein levels of PC3 cells in 200 mg/L group were decreased(P＜0.05).By molecular docking prediction analysis,sophoridine may interact with Gln407,Lys233,Arg469,Lys180,Ser411 and Asp412 on β-Catenin protein.Conclusion Sophoridine may interact with β-Catenin protein to inhibit the expression ofβ-Catenin,CyclinD1 and MMP9 proteins in PC3 cells,thereby affecting the activity of Wnt/β-Catenin pathway and inhibiting the growth and invasion of cancer cells.Therefore,sophoridine plays a role in cancer inhibition in prostate cancer.
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