Effect of high expression of PNPLA3 gene on cell proliferation and triglyceride in model of nonalcoholic fatty liver disease
Objective To investigate the effect of high expression of patatin-like phospholipase domain 3(PNPLA3)on proli-feration and triglyceride(TG)of nonalcoholic fatty liver disease(NAFLD)model cells.MethodsHuman hepatoma cells(SMMC-7721 cells)were divided into control group,model group,PNPLA3 overexpression group,and PNPLA3 overexpression noload group.Model group:NAFLD cell model was established by free fatty acid induction,and PNPLA3 overexpression group and PNPLA3 overexpress noload group obtained by lentivirus stock solution infection and puromycin screening after modeling.Quantitative reverse transcription polymerase chain reaction(qRT-PCR),MTT colorimetric assay,and TG testing were used to detect the expression of PNPLA3,cell viability,and intracellular TG content in cells.Results The results of qRT-PCR showed that the expression level of PNPLA3 gene in the model group was higher than that in the control group(P＜0.01),and the expression level of PNPLA3 gene in the PNPLA3 overexpression group was higher than that in the model group(P＜0.01).MTT results showed that the relative survival rate of cells in the model group was higher than that in the control group(P＜0.05),and the relative survival rate of cells in the PNPLA3 overexpression group was higher than that in the model group(P＜0.01).The results of TG detection showed that TG content in model group was higher than that in control group(P＜0.01),the TG content in the PNPLA3 overexpression group was higher than that in the model group(P＜0.05).Conclusion The stable NAFLD strain with PNPLA3 overexpression is successfully constructed,and the over-expression of PNPLA3 contributes to the accumulation of lipid and increases the relative survival rate of cells.
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