Effect of serum exosomes from obstructive sleep apnea-hypopnea syn-drome patients on growth and invasion of A549 cells
Objective To investigate the effect of serum exosomes from obstructive sleep apnea-hypopnea syndrome(OSAHS)patients on growth and invasion of A549 lung cancer cells.Methods A total of 21 patients with OSAHS admitted to the First Affiliated Hospital of Xinjiang Medical University from August 2023 to February 2024 were selected as the experimental group,and 11 healthy volunteers who underwent physical examinations were selected as the control group.Their serum and extracted serum exosomes from OSAHS patients and healthy volunteers were collected.OSAHS patients'serum exosomes co-cultured with A549 cells were regarded as OSAHS group,and healthy volunteers'serum exosomes co-cultured with A549 cells were regarded as HC group.CCK assay was used to detect tumor cell activity.Transwell assay and cell scratch assay were used to detect the invasion and migration ability of A549 cells.The AnnexinV-PE/TAAD apoptosis detection kit was used to detect the apoptosis of A549 cells.Western blot was used to detect the expression levels of E-cadherin,N-cadherin,and matrix metalloproteinase 9(MMP-9)in A549 cells.Results Compared with the HC group,the cell viability of OSAHS group was increased(P＜0.01).Compared with the HC group,the invasion and migration ability of A549 cells were increased by serum exosomes in OSAHS group(P＜0.01).The expression level of MMP-9 in OSAHS group was higher than that in HC group(P＜0.05).The apoptosis rate of A549 cells in the OSAHS group was lower than that in the HC group(P＜0.05).Conclusion Serum exosomes from OSAHS patients may promote the growth and invasion of A549 human lung cancer cells.
Obstructive sleep apnea-hypopnea syndromeA549 lung cancer cellsGrowthInvasiveness
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