Determination of 17 Amino Acids in Human Plasma by High Performance Liquid Chromatography with Pre-column Derivatization
Objective To detect 17 amino acids in human plasma by HPLC with pre-column derivatization using 6-amin-oquinolyl-N-hydroxysuccinimidyl carbamate (AQC). Methods Plasma samples were pretreated with AQC as derivatization a-gent. The amino acid derivatives were separated on a C18 column with fluorescence detection (λex = 250 nm, λem = 395 nm) after gradient elution. The method was applied to determine the concentration of 17 free amino acids in human plasma from 22 healthy volunteers. Results The analytes displayed a good linearity over the tested concentration range. The average recovery was within the range of (87. 7±5. 8)%-(117. 1±8.4)%. The intra- and inter-variation coefficients were less than 10.3% and 11.0% , respectively. All tested amino acids in the plasma samples are within the normal reference range. Conclusion The method is simple and stable with decent separation, which can be generally used to determine amino acids in human plasma.
国家科技期刊平台
NSTL
万方数据
维普
