Construction and efficiency analysis of denitrifying desulfurizing compound bacteria in shrimp culture environment
With the rapid development of intensive culture of shrimp,the pursuit of high yield coupled with excessive feeding leads to the aggravation of NH4+-N,NO2--N,and sulfide pollution in the aquaculture water,which seriously harms the growth and physiological function of shrimp.This study used heterotrophic nitrification-aerobic denitrifying bacteria(HHVEN1 and SDVEA2)and sulfur-oxidizing bacteria(GHWS3 and GHWS5)screened from shrimp aquaculture environment to successfully construct two efficient denitrification/desulfurization bacterial consortia NS1-1 and NS2-2,and explored their denitrification and desulfurization capabilities.The results showed the NS1-1 consortium displayed removal rates exceeding 95％for NH4+-N,N02--N,and sulfide,and NS2-2 exhibited removal rates of 90.27％,97.38％,and 89.62％,respectively,all of which were superior to a single strain.The bacterial consortia NS1-1 and NS2-2 can exert maximum denitrification/desulfurization efficiency under the culture conditions of temperature 20-35 ℃,carbon-nitrogen ratio(C/N)5-20,salinity 20-30,and utilizing glucose or sodium succinate as the carbon source.The optimum pH ranges of the two consortia were different,with NS1-1 favoring 7.5-8.0 and NS2-2 preferring 7.0-8.5.The bacterial consortia NS1-1 and NS2-2 had good coexistence among various strains in aquaculture tailwater.NS1-1 and NS2-2 displayed effective removal rates of 78.75％,75.09％,79.61％,and 81.44％,62.68％,72.64％for NH4+-N,NO2--N-and sulfide in practical aquaculture tailwater,respectively.The research shows that the bacterial consortia NS1-1 and NS2-2 have high-efficiency denitrification desulfurization removal efficiency and exceptional environmental adaptability,providing scientific support for eliminating nitrogen and sulfur pollution in aquaculture.
万方数据
维普
