摘要
随着对虾集约化养殖的快速发展,由于追求高产量和过量投饲等原因导致养殖水体中氨氮(NH4+-N)、亚硝酸盐氮(NO2--N)和硫化物污染加重,严重危害对虾生长和生理功能.本研究利用自对虾养殖环境中筛选出的异养硝化-好氧反硝化菌(HHVEN1和SDVEA2)和硫氧化菌(GHWS3和GHWS5),成功构建了两种高效脱氮脱硫复合菌NS1-1和NS2-2,并研究其脱氮、脱硫能力.结果显示:复合菌NS1-1对NH4+-N、NO--N和硫化物去除率均达到95％以上,NS2-2的去除率分别为90.27％、97.38％和89.62％,且均优于单一菌株;复合菌NS1-1和NS2-2在温度20℃～35℃、碳氮比(C/N)5～20、盐度20～30和以葡萄糖或琥珀酸钠为碳源的培养条件下均能发挥最大脱氮脱硫效能,两者最适pH范围不同,分别为7.5～8.0和7.0～8.5.复合菌NS1-1和NS2-2在水产养殖尾水中各菌株之间具有良好的共存性,在实际养殖尾水中NH4+-N、NO2--N、硫化物去除率分别为78.75％、75.09％、79.61％和81.44％、62.68％、72.64％.研究表明,复合菌NS1-1和NS2-2具有高效脱氮脱硫的去除效能和优异的环境适应能力,研究成果为水产养殖环境消除氮、硫污染提供了科学支持.
Abstract
With the rapid development of intensive culture of shrimp,the pursuit of high yield coupled with excessive feeding leads to the aggravation of NH4+-N,NO2--N,and sulfide pollution in the aquaculture water,which seriously harms the growth and physiological function of shrimp.This study used heterotrophic nitrification-aerobic denitrifying bacteria(HHVEN1 and SDVEA2)and sulfur-oxidizing bacteria(GHWS3 and GHWS5)screened from shrimp aquaculture environment to successfully construct two efficient denitrification/desulfurization bacterial consortia NS1-1 and NS2-2,and explored their denitrification and desulfurization capabilities.The results showed the NS1-1 consortium displayed removal rates exceeding 95％for NH4+-N,N02--N,and sulfide,and NS2-2 exhibited removal rates of 90.27％,97.38％,and 89.62％,respectively,all of which were superior to a single strain.The bacterial consortia NS1-1 and NS2-2 can exert maximum denitrification/desulfurization efficiency under the culture conditions of temperature 20-35 ℃,carbon-nitrogen ratio(C/N)5-20,salinity 20-30,and utilizing glucose or sodium succinate as the carbon source.The optimum pH ranges of the two consortia were different,with NS1-1 favoring 7.5-8.0 and NS2-2 preferring 7.0-8.5.The bacterial consortia NS1-1 and NS2-2 had good coexistence among various strains in aquaculture tailwater.NS1-1 and NS2-2 displayed effective removal rates of 78.75％,75.09％,79.61％,and 81.44％,62.68％,72.64％for NH4+-N,NO2--N-and sulfide in practical aquaculture tailwater,respectively.The research shows that the bacterial consortia NS1-1 and NS2-2 have high-efficiency denitrification desulfurization removal efficiency and exceptional environmental adaptability,providing scientific support for eliminating nitrogen and sulfur pollution in aquaculture.
基金项目
广东省重点领域研发计划项目(2020B0202010009")
维普
万方数据