Anti-Inflammatory Mechanism of Paris Saponin Ⅶ via NF-κB Signaling Pathway
Objective To decipher the anti-inflammatory mechanism of Paris saponin Ⅶ(PSⅦ).Methods Naive T lym-phocytes were isolated from the spleen of C57BL/6 mice by magnetic-activated cell sorting.After Paris saponin Ⅶ treat-ment,flow cytometry,RT-qPCR and Western blotting were employed to examine the effects of PSⅦon the expression lev-el of interleukin-6(IL-6).The effects of PSⅦon the activation,proliferation and differentiation of CD4+T cells were de-tected by flow cytometry and Cell TraceTM Violet kit.Western blot was used to determine the expression and phosphoprotein of nuclear factor-kappa beta inhibitor alpha(IκBα)and signal transducer and activator of transcription 3(STAT3)after PSⅦ treatment.The differentially expressed genes(DEGs)after PSⅦ treatment were detected by RNA-seq.Results PSⅦ significantly inhibited the expression of IL-6.PSⅦ had no obviously effect on the activation and proliferation,but can influenced the differentiation of CD4+T cells.PSⅦcan inhibited the phosphoprotein of IκBα and STAT3,but had no effect on the expression of the two proteins.A total of 70 DEGs were detected by RNA-seq,including 27 up-regulated genes and 43 down-regulated genes,from which 6 genes were selected and verified by RT-qPCR.Conclusion PSⅦ can relieve in-flammation by inhibiting IL-6 expression via STAT3 and NF-κB signaling pathways.
Paris saponin ⅦNF-κB signaling pathwayIL-6Inflammation
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