药用植物冷水花DNA条形码分析和基因组大小测定
DNA Barcoding and Genome Size Estimation of the Medicinal Plant Pilea notata
罗湘胤 1游磊 1叶峥秀 1张泽志 2石宪铭 1居婷婷 1李琳 1张勇洪1
作者信息
- 1. 湖北医药学院基础医学院药用植物及进化遗传学实验室;武当特色中药研究湖北省重点实验室
- 2. 十堰市农业科学院,湖北 十堰 442000
- 折叠
摘要
目的:鉴定采集到的冷水花属植物样本并测定其基因组大小.方法:用聚合酶链式反应扩增样品的ITS2、rbcL和psbA-trnH序列并测序,通过比对Nr核酸数据库确定物种;以玉米Mo17 为对照,用流式细胞术测定其基因组大小.结果:本研究采集到的冷水花植物与冷水花(Pilea notata)ITS2 序列一致性为 100%,其基因组大小约为 498.4 Mb.结论:ITS2 序列可以较好地区分冷水花属植物,采用流式细胞术可以简便地估算待测植物样品的基因组大小.
Abstract
Objective To identify the collected Pilea L.plant sample and estimate its genome size.Methods PCR amplifica-tion and sequencing were performed for the ITS2,rbcL and psbA-trnH sequences,and then sequence alignments against the Nr database were performed for species identification.With the maize Mo17 genome as the control,flow cytometry was em-ployed to estimate the genome size of this plant.Results The ITS2 sequence of collected sample showed the identity of 100%to that of Pilea notata,with an estimated genome size of 498.4 Mb.Conclusion ITS2 sequences can distinguish dif-ferent species of Pilea and flow cytometry is a convenient way to estimate plant genome size.
关键词
冷水花/DNA条形码/流式细胞术/基因组大小Key words
Pilea notata/DNA barcoding/Flow cytometry/Genome size引用本文复制引用
基金项目
国家自然科学基金(32000609)
湖北省教育厅项目(B2021163)
湖北省教育厅项目(Q20222106)
出版年
2024
国家科技期刊平台
NSTL
万方数据