首页|蛋白磷酸酶PPM1F促进非小细胞肺癌的进展

蛋白磷酸酶PPM1F促进非小细胞肺癌的进展

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的:探讨蛋白磷酸酶PPM1F对非小细胞肺癌增殖和迁移的影响.方法:构建PPM1F敲低载体,通过Western blotting 验证PPM1F的敲低效率.通过CCK-8 实验、克隆形成实验、迁移实验、侵袭实验和流式细胞术检测PPM1F对非小细胞肺癌细胞功能的影响.结果:成功构建了PPM1F敲低质粒.敲低PPM1F抑制了非小细胞肺癌生长、增殖、迁移和侵袭能力.同时敲低 PPM1F,非小细胞肺癌细胞凋亡率增加,细胞在 G1 期累积.结论:PPM1F在非小细胞肺癌中以癌基因的作用发挥功能.
The Protein Phosphatase PPM1F Promotes the Progression of Non-Small Cell Lung Cancer:a Review
Objective To investigate the effects of the protein phosphatase PPM1F on the proliferation and metastasis of non-small cell lung cancer(NSCLC).Methods The vector for PPM1F knockdown was constructed,and Western blotting was employed to verify the knockdown efficiency of PPM1F.The effect of PPM1F on the function of NSCLC cells was deter-mined by the CCK-8 assay,colony formation assay,Transwell assay,invasion assay and flow cytometry.Results The plas-mid for PPM1F knockdown was successfully constructed.PPM1F knockdown inhibited the growth,proliferation,migration and invasion of NSCLC cells.When PPM1F was knocked down,the apoptosis rate of NSCLC cells increased,and the cells accumulated in the G1 phase.Conclusion PPM1F plays a role of oncogene in NSCLC.

Protein phosphatasePPM1FNon-small cell lung cancer

周阳、刘家琪、柳淞、李子航、马永清、冯文筱、潘中秋、张慧美、赵红艳

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湖北医药学院基础医学院胚胎干细胞研究湖北省重点实验室,湖北 十堰 442000

蛋白磷酸酶 PPM1F 非小细胞肺癌

湖北省科技厅基金重大项目大学生创新创业训练项目

2022BCE012X202210929011

2024

10.13819/j.issn.2096-708X.2024.01.004

湖北医药学院学报
湖北医药学院

湖北医药学院学报

影响因子：0.504
ISSN：1006-9674
年,卷(期)：2024.43(1)
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