Determination of Vitamin C in Clinacanthus Nutans Content by High Performance Liquid Chromatography
Objective To establish a high performance liquid chromatography(HPLC)-based method for the determination of vitamin C in Clinacanthus nutans.Methods In this study,a method for the determination of vitamin C was established based on high performance liquid chromatography(HPLC),and the standard curve was established with the standard sub-stance.The linear relationship,detection limit,quantitative limit,repeatability,precision and standard addition recovery of the method were investigated.Results The established method had a good linearity within the range of 0.1 to 50.0 μg/mL,with the regression equation of y=53017x+2630.9,R2=0.999,the limit of detection of 0.06 μg/mL,the limit of quantifi-cation of 0.21 μg/mL,the relative standard deviation(RSD)of 3.80%in repeatability,the RSD of 1.49%in precision and the recovery rate of 99.50%to 102.97%.Conclusion The established method is suitable for determining the vitamin C in C.nutans,providing a reference for the development and utilization of C.nutans resources.
国家科技期刊平台
NSTL
万方数据
