Study on the Correlation Between the Expression Changes of miR-223 and miR-33a/b in HDL and the Degree of Coronary Artery Stenosis
Objective To investigate the correlation between the expression levels of miR-223,miR-33a and miR-33b in high density lipoprotein(HDL)isolated from plasma and the degree of coronary artery stenosis.Methods A total of 86 pa-tients who were admitted to the cardiology department of our hospital for chest tightness,chest pain and palpitation were se-lected to undergo coronary angiography(CAG)and divided into control group and coronary heart disease(CAD)group ac-cording to CAG results.The CAD group CAD group further categorized into mild-to-moderate stenosis group and severe stenosis group based on Gensini scores.HDL was extracted from plasma by ultracentrifugation,and the expression levels of miR-223,miR-33a and miR-33b in HDL of each group were detected by real-time fluorescent quantitative PCR.Multi-variate Logistic regression was used to analyze the influencing factors of CAD,and Spearman correlation analysis was used to analyze the correlation between miRNA expression in HDL and Gensini score in CAD patients.ROC was also plotted to e-valuate the value of HDL miRNA expression in CAD diagnosis.Results The expression level of HDL-miR-223 in the CAD group was significantly higher than that in the control group,while the expression level of HDL-miR-33a was lower than that in the control group.Additionally,in the CAD severe stenosis group,the relative expression level of HDL-miR-223 was higher than that in the mild-to-moderate stenosis group.The relative expression level of HDL-miR-223 in CAD pa-tients was positively correlated with Gensini scores.ROC analysis demonstrated that both HDL-miR-223 and HDL-miR-33a had certain predictive value for CAD diagnosis,and their combination had a higher predictive value for diagnosing CAD.Conclusion miR-223 and miR-33a in HDL are closely related to the occurrence and development of CAD,and the expression of miR-223 in HDL increases with the progression of coronary artery stenosis.
Coronary heart diseaseHDLmiRNADegree of coronary artery stenosis
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