Research on the Mechanism of Kanglixin Capsule in the Treatment of Breast Cancer Based on GEO Chip and Net-work Pharmacology
Objective To investigate the mechanism of Kanglixin capsule in breast cancer based on GEO chip and network pharmacology.Methods The chemical ingredients of drugs were collected by TCMSP,HERB and Batman-TCM database,while Swiss Target Prediction was used to predict the corresponding targets of ingredients.The targets of breast cancer were retrieved in GEO database.Venny 2.1.0 online software was used to obtain the common targets of drugs-disease,and then the "drug-compound-target-disease" net-work diagram was constructed by using the software Cytoscape 3.8.2.The STRING database was used to draw the PPI network,and the perform GO function and KEGG pathway enrichment analysis were carried out through DAVID database,and the AutoDock platform was applied in verifying the molecular docking of ac-tive components and protein targets.Results The 59 active components of Kanglixin capsule could regulate 106 targets and 43 pathways to treat breast cancer.Farnesiferol A,gummosin,conferone,kaempferol,farnesif-erol B,EUPATIN,quer-cetin,conferol,cerevisterol,assafoetidin and other active compounds,which could mediate central carbon metabolism in cancer,cell cycle,metabolic pathways,pathways in cancer,AMPK,relaxin,FoxO,IL-17 Ras,HIF-1,MAPK,calci-um,insulin and other signaling pathways through EGFR,JUN,PPARG,PTGS2,CCNA2,CHEK1,CDK1,CDK2,MMP9,TOP2A and other key target proteins.Molecular docking analysis showed that 10 target proteins had good binding ability with 10 active components.Conclusion Kanglixin capsule can participate in the treatment of breast cancer through multiple components,targets and pathways,which provides a theoretical basis for the clinical application of Kanglixin cap-sule and the mechanism of its treatment of breast cancer.
Kanglixin capsuleBreast cancerGEO chipNetwork pharmacologyMolecular dockingMechanism of ac-tion
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