对羟基苯甲酸乙酯暴露于雌性斑马鱼对子代胚胎发育的毒性效应
Toxic Effect of Ethylparaben Exposure on the Embryonic Development of the Offspring of Female Zebrafish
廖秋月 1李鑫 1孔伟蒙 1于鹏霞 1黄娇龙 2段鹏 1廉凯 2邓杰1
作者信息
- 1. 湖北医药学院附属襄阳市第一人民医院妇产科;湖北省胎儿复杂畸形精确诊断临床医学研究中心;人类疾病斑马鱼模型新药筛选襄阳市重点实验室,湖北 襄阳 441000
- 2. 湖北医药学院附属襄阳市第一人民医院妇产科;人类疾病斑马鱼模型新药筛选襄阳市重点实验室,湖北 襄阳 441000
- 折叠
摘要
目的:探讨对羟基苯甲酸乙酯(EtP)暴露对子一代(F1)斑马鱼胚胎发育毒性的影响及其相关分子机制.方法:将四月龄的雌性斑马鱼持续暴露于文献报道相关浓度 0.5 mg/L和 2mg/L中 28 d,染毒结束后,将已暴露EtP的雌性斑马鱼与同周龄的雄性斑马鱼交配,观察F1 代胚胎发育情况,采用转录组测序技术(RNA-seq),进一步阐释EtP暴露对F1 代毒性效应的分子机制.结果:与对照组比较,EtP组F1 代 96 hpf孵化率明显降低;死亡率和畸形率明显增加(P＜0.05),96 hpf卵黄囊面积和心率增加,24 hpf自发卷尾运动明显增加(P＜0.05);96 hpf眼面积明显减小(P＜0.05).EtP组F1 代 144 hpf幼鱼运动距离和游泳速度均明显降低(P＜0.05).F1 代 96 hpf幼鱼RNA-seq测序结果表明,DEGs与神经系统发育调节、眼睛的视网膜发育有关,且RT-qPCR与RNA-seq结果一致.结论:EtP母源性暴露可导致无EtP直接暴露的F1 代斑马鱼胚胎发育异常,特别是神经系统发育和视网膜发育.
Abstract
Objective To understand the toxic effect of ethylparaben(EtP)exposure on the embryonic development of the F1 generation of zebrafish and to decipher the underlying molecular mechanisms.Methods Four-month female zebrafish were continuously exposed to 0.5 mg/L and 2 mg/L EtP for 28 days.After exposure,these females were mated with age-matched males.The embryonic development of the F1 generation was observed,and RNA sequencing(RNA-seq)was uti-lized to reveal the molecular mechanisms of EtP exposure on the toxic effect in the F1 generation.Results Compared with the control group,the F1 generation at 96 hpf in the EtP group displayed a significant reduction in hatching rate,an in-crease in mortality and malformation(P＜0.05),an enlargement of the yolk sac area at 96 hpf,an elevation in heart rate,and an increase in spontaneous tail-curling motion at both 96 hpf and 24 hpf(P＜0.05),as well as a noticeable reduction in eye size at 96 hpf(P＜0.05).Additionally,at 144 hpf,larval swimming distance and speed were decreased(P＜0·05).RNA-seq for the F1 generation at 96 hpf showed that differentially expressed genes(DEGs)were associated with the regu-lation of nervous system development and retina formation,which is consistent with the results of RT-qPCR.Conclusion EtP exposure in female zebrafish adversely affects embryonic development,particularly impacting nervous system develop-ment and retina formation,in their offspring,even without direct EtP exposure.
关键词
对羟基苯甲酸乙酯/雌性斑马鱼/胚胎发育Key words
Ethylparaben/Female zebrafish/Embryonic development引用本文复制引用
基金项目
国家自然科学基金(82301938)
湖北省重点研发计划(2022CFD010)
湖北省重点研发计划(2022BCE021)
湖北省"323"攻坚行动襄阳市第一人民医院重点专项(XYY2022-323)
出版年
2024
国家科技期刊平台
NSTL
万方数据