Experimental Study of Rhopaladins Analogue RPDPRH Against Hepatoma Cell Proliferation
Objctive To examine the effects of the analogue of marine alkaloids Rhopaladins(2E,4E)-4-(4-chloro-benzylidene)-2-(4-chlorostyryl)-N-cyclohexyl-1-(4-fluorophenyl)-5-oxopyrrplidine-2-carboxamide(RPDPRH)on proliferation inhibition and cycle arrest of human hepatoma HepG2 and Bel-7402 cell lines.Methods Growth inhibition by RPDPRH on HepG2 and Bel-7402 cells was assessed by a CCK-8 assay.The effects on colony formation of these cells were evaluated,along with cell cycle analysis to understand the potential blockage induced by RPDPRH.Hoechst 33258 staining was employed to observe the apoptotic changes in treated cells under a fluorescence microscope.Results CCK-8 results indicated that RPDPRH had a modest inhibitory effect on the proliferation of liver LO2 cells,while it significantly suppressed the proliferation of HepG2 and Bel-7402 cells.Colony formation assay and cell cycle analysis confirmed that RPDPRH inhibited colony formation in HepG2 and Bel-7402 cells and arrested the cell cycle in the G0/G1 phase.Hoechst 33258 staining revealed significant hepatic cell apoptosis after RPDPRH intervention.Conclusion RPDPRH shows minimal inhibition on LO2 cells but exhibits potent anti-proliferative effects on HepG2 and Bel-7402 cell lines,which may be relat-ed to the arrest of cell cycle in the G0/G1 phase.
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