DNA Barcoding and Genome Size Estimation of Medicinal Plant Saxifraga stolonifera
Objective To comprehensively explore the genetic landscape of the plant resources of Saxifraga stolonifera via i-dentifying its species and determining its genome size.Methods Screening the DNA barcode primers for Saxifraga stolonif-era revealed that he ITS2+matK barcode can confirm the tested plant sample as Saxifraga stolonifera.Furthermore,a phylo-genetic tree was constructed by using the ITS2 and matK barcode sequences of S.stolonifera and its closely related species.Evolutionary analysis results indicated that S.stolonifera had a distant phylogenetic relationship with other species in the same genus.Results Flow cytometry estimated the genome size of S.stolonifera is approximately 949.4 Mb.Conclusion This study utilized barcode sequences to identify the species of Saxifraga stolonifera native to Wudang Mountain in Shiyan,providing characteristic information for its genome.Our work may serve as a reference for species selection and sequencing strategy planning for subsequent whole-genome sequencing studies of S.stolonifera.
国家科技期刊平台
NSTL
万方数据
