Endogenous Metabolic Changes and Disease Mechanisms in Chronic Colitis Rats Based on Metabolomic Analysis
Objective To evaluate the changes in endogenous metabolites in rats with trinitrobenzene sulfonic acid-induced chronic colitis using nuclear magnetic resonance(NMR)and to elucidate the underlying mechanisms of chronic colitis.Methods Thirty SD rats were randomly assigned to a chronic colitis group(CC group)and a normal control group(NC group).Serum levels of myeloperoxidase(MPO),interleukin-2(IL-2),and interleukin-4(IL-4)were quantified u-sing enzyme-linked immunosorbent assay(ELISA).1 H NMR was used to measure the endogenous metabolites in rat urine and fecal samples.Results Compared with the NC group,the CC group exhibited increased MPO and IL-2,but decreased IL-4,along with up-regulation of branched-chain amino acids(leucine and isoleucine),glycine and phenylpropyl amino acids in urine NMR,while significant down-regulation of fumaric acid,taurine,hippuric acid and acetoacetic acid.In fe-cal samples from the CC group,glycerophospholipid choline and serine were significantly reduced,while uracil and creatine were markedly elevated,mainly involving amino acid and lipid metabolism.Conclusion Chronic colitis is related to the dis-order of amino acid and lipid metabolism,and the mechanism of which may be associated with the inhibition of amino acid metabolic pathways and the abnormal metabolism of glycerophospholipids.
MetabolomicsNuclear magnetic resonance(NMR)Chronic colitis
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