Analysis of Potential Prognostic Biomarker HROB in Lung Adenocarcinoma Based on Bioinformatics
Objective To analyze the expression of HROB in lung adenocarcinoma(LUAD)and its correlation with progno-sis based on bioinformatics.Methods Gene expression profiles and clinical data of LUAD patients were downloaded from the TCGA database.The immunohistochemical data of LUAD patients and healthy individuals were obtained from the HPA database.R software was used to analyze the differential expression of HROB between tumor and adjacent tissues.Clinical correlation analysis revealed the association between HROB and clinicopathological features of LUAD patients.Kaplan-Meier survival analysis combined with ROC curve was employed to evaluate the diagnostic value and prognosis of HROB.Cox regression analysis was conducted to identify the independent prognostic factors,and GO/KEGG and GSEA were per-formed to explore the potential functions of HROB in LUAD.The correlation between HROB and tumor-infiltrating immune cells was assessed using CIBERSORT.Results HROB was higher in LUAD tissues than in adjacent normal tissues(P＜0.05)and its expression was positively correlated with age,smoking,initial treatment effect,TNM stage,pathological grade,overall survival(OS),disease-specific survival(DSS),and progression-free interval(PFI)(P＜0.05).Survival analysis showed that LUAD patients with low HROB had higher OS,PFI,and DSS than those with high HROB(P＜0.05).Cox regression analysis revealed that pathological gradesⅢandⅣwere independent risk factors for the prognosis of LUAD(P＜0.05),while high HROB could not be regarded an independent prognostic factor(P＞0.05).Function enrichment a-nalysis suggested that HROB was primarily involved in cell cycle,chromosome segregation,and homologous recombination.In the immune cell infiltration of LUAD,there was a positive correlation between high HROB and NK CD56dim,Tgd,and Th2 cells(P＜0.05).Conclusion High HROB is closely associated with progression,prognosis,and immune regulation of LUAD patients,indicating its potential as a prognostic biomarker and therapeutic target for LUAD.
万方数据
维普
