Intestinal Absorption of Anemarrhena asphodeloides Extract Based on Single-pass Intestinal Perfusion Model
Objective To study the absorption of seven active components of Anemarrhena asphodeloides extract in the intes-tinal tract of rats and to elucidate the intestinal absorption mechanism of the active components.Methods The rat single-pass intestinal perfusion(SPIP)model was used to analyze the intestinal absorption of the seven active components.The in-testinal perfusion samples were processed by solid-phase extraction.The absorption changes of multiple active components of A.asphodeloides was quantitative analyzed using LC-MS/MS.Results The absorption constants(Ka)of mangiferin,neomangiferin,timosaponin BⅡ,baohuosideⅠ,and sarsasapogenin showed differences(P＜0.05),indicating that the main absorption site of these substances was in the jejunum.The effective permeability(Peff)of the main active components was all＞1.0×10-3 cm/min,suggesting good absorption in the duodenum and jejunum of rats.Conclusion The main ab-sorption site of mangiferin,neomangiferin,timosaponin BⅡ,baohuoside Ⅰ,and sarsasapogenin is in the jejunum.
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