Optimized Preparation of G-banding Chromosomes from Human Peripheral Blood Lymphocytes with Different Culture Duration
Objective To explore the appropriate methods for obtaining G-banding chromosomes from human peripheral blood lymphocytes with different culture duration for clinical diagnosis.Methods Peripheral blood was cultured for 68～72 h as the control group.Four experimental groups were set up according to the culture duration of 24 h,48 h,96 h,and 120 h.Three rounds of comparative experiments were conducted on the factors of culture duration time,colchicine concentra-tion,and cell concentration.The division index and the effect of chromosome G-banding in different experimental groups were compared to find the optimal experimental conditions for groups with different culture duration.Results There was no significant difference between the experimental groups ( 48 h culture,22 drops of peripheral blood lymphocytes,colchicine concentration of 0.14 μg/mL and 96 h culture,20 drops of peripheral blood lymphocytes,colchicine concentration of 0.06μg/mL) and the control group.Conclusion The desirable G-banding after 68～72 h culture and analysis results can be a-chieved by adjusting cell concentration and colchicine concentration.By this method,specimens can be flexibly processed in batches,which greatly improves the efficiency of laboratory detection in clinical practice.
万方数据
维普
