Effect of Peritoneal Injection of Pyruvate Peritoneal Dialysis Solution on Abdominal Organs in Septic Shock Rats
Objective To observe the effect of peritoneal injection of pyruvate peritoneal dialysis solution on the abdominal organs in septic shock rats.Methods Fifty-four healthy male SD rats were used to replicate a septic shock model by intra-venous injection of endotoxins.The 45 successfully modeled rats were randomly divided into three groups:single intrave-nous resuscitation ( group A),intravenous resuscitation+peritoneal injection of lactate peritoneal dialysis solution ( group B),and intravenous resuscitation+peritoneal injection of pyruvate peritoneal dialysis solution ( group C) .Intravenous resus-citation was performed by rapid intravenous infusion of Ringer's solution at 25 mL/kg for 30 min,followed by peritoneal in-jection of lactate peritoneal dialysis solution at 100 mL/kg for 20 min in group B,or an equal amount of pyruvate peritoneal dialysis solution in group C.After 20 h of resuscitation,the survival status of the rats was recorded.Changes in the abdomi-nal organs of the survived rats were observed anatomically,and the liver and ileum tissues were collected to determine the dry wet weight ratio,MDA content,MPO activity,and TNF-α.Histopathological sections were stained with HE.Results The survival rate of rats in groups B and C was higher than that of group A after resuscitation for 20 h,while that of group C was higher than that of group B ( P<0.05) .The MDA content,MPO activity,TNF-α,tissue edema,and morphological damage in the liver and ileum tissues of rats in groups B and C were lower than those of group A,and group C was lower than group B in the above indexes (P<0.05).Conclusion Peritoneal injection of pyruvate peritoneal dialysis solution in-hibits lipid peroxidation and inflammatory reactions in the abdominal organs of septic shock rats and thus reduces the dam-age to the abdominal organs,which improves the treatment effectiveness of shock.
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