In this research,the molecular mechanism of"Huangqi Bufei Yin"regulating oxidative stress was studied using network pharmacology and molecular docking.The main active ingredients(kaempferol,querce-tin and stigmasterol)and core targets(AKT1,MMP9 and CASP3)were screened out.Through Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis,the role of these components in the antioxidative stress process of"Huangqi Bufei Yin"was inferred at the molecular lev-el.The molecular docking results showed that the active ingredients could bind to the targets to form stable conformations,revealing the characteristics and advantages of"Huangqi Bufei Yin"in terms of the multi-com-ponent,multi-target and multi-pathway.In addition,in vitro antioxidant experiment based on the combination index indicated that the optimal ratio of Mume fructus,Hedysarum multijugum maxim,Schisandrae chinensis fructus and Ophiopogon japonicus was 4∶1∶1∶4,showing the highest scavenging rate and best synergistic effect.Using 3D printing technology,the optimally ratioed of"Huangqi Bufei Yin"was made into a gel,im-proving its convenience and slow-release ability,providing a new idea for the large-scale production of tradi-tional diet therapy.
关键词
黄芪补肺饮/氧化应激/网络药理学/分子对接/抗氧化能力/联合指数
Key words
"Huangqi Bufei Yin"/oxidative stress/network pharmacology/molecular docking/antioxidant capacity/combination index
万方数据