首页|Downregulation of cold-inducible RNA-binding protein activates mitogen-activated protein kinases and impairs spermatogenic function in mouse testes

Downregulation of cold-inducible RNA-binding protein activates mitogen-activated protein kinases and impairs spermatogenic function in mouse testes

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Cold-inducible RNA-binding protein (CIRP) is an RNA-binding protein that is expressed in normal testes and downregulated after heat stress caused by cryptorchidism,varicocele or environmental temperatures.The purpose of this study was to investigate the functions of CIRP in the testes.We employed RNAi technique to knock down the expression of CIRP in the testes,and performed haematoxylin and eosin staining to evaluate morphological changes following knockdown.Germ cell apoptosis was examined by terminal deoxynucleotidal transferase-mediated dUTP nick end labelling (TUNEL) assay,and mitogen-activated protein kinase (MAPK)signalling pathways were investigated by Western blotting to determine the possible mechanism of apoptosis.We found that using siRNA is a feasible and reliable method for knocking down gene expression in the testes.Compared to controls,the mean seminiferous tubule diameter (MSTD) and the thickness of the germ cell layers decreased following siRNA treatment,whereas the percentage of apoptotic seminiferous tubules increased.The p44/p42,p38 and SAPK/JNK MAPK pathways were activated after downregulation of CIRP.In conclusion,we discovered that downregulation of CIRP resulted in increased germ cell apoptosis,possibly viathe activation of the p44/p42,p38 and SAPK/JNK MAPK pathways.

cold-inducible RNA-binding protein (CIRP)mitogen-activated protein kinase (MAPK)siRNA in vivospermatogenesisheat stressmale infertility

Zhi-Ping Xia、Xin-Min Zheng、Hang Zheng、Xiao-Jun Liu、Gui-Yong Liu、Xing-Huan Wang

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Department of Urology, Zhongnan Hospital of Wuhan University, Wuhan 430071, China

Department of Urology, Qianjiang Central Hospital, Qianjiang 433100, China

Fundamental Research Funds for the Central UniversitiesZhongnan Hospital of Wuhan University support

20113030202001363

2012

10.1038/aja.2012.71

亚洲男性学杂志(英文版)
中国科学院上海药物研究所

亚洲男性学杂志(英文版)

CSTPCDCSCDSCI
影响因子：0.514
ISSN：1008-682X
年,卷(期)：2012.14(6)
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