Jatonik polyherbal mixture induced rat liver MMPT pore opening in normal Wistar rat:In vitro and in vivo studies

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外文摘要：Objective:To assess acute toxicity,the in vitro and in vivo effects of methanol and ethyl acetate extracts(JME and J EE)of Jatonik polyherbal mixture on some mitochondria-related parameters and their effect on the activity of some liver enzymes.Methods:Acute toxicity of JME and JEE was determined using Lorke's method.In vitro and in vivo opening of the mitochondrial membrane permeability transition pore(MMPT pore)was spectrophotometrically assayed.Production of malondialdehyde(MDA)as an index of lipid peroxidation and the activity of mito-chondrial ATPase was evaluated in vitro and in vivo and the effect of JME and JEE on the activity of liver enzymes such as alkaline phosphatase(ALP),aspartate and alanine aminotransferase(AST and ALT)and gamma-glutamyl transferase(GGT)was also investigated.Results:JME had an LD50 of 3 808 mg/kg b.w whereas JEE had an LD50 greater than 5 000 mg/kg b.w.of rats.After the rats have been fed with both extracts,a photomicrograph of a piece of liver tissue showed no apparent symptoms of toxicity.From the in vitro and in vivo studies,both extracts prompted intact mitochondria to open their MMPT pores.When compared to the control,lipid peroxide product release and ATPase activity were significantly increased(P＜0.05)in vitro and in vivo.The activities of AST,ALT,and GGT were all reduced at 50 mg/kg when treated with JME,but the activity of AST was considerably enhanced when treated with JEE(P＜0.05).The results revealed that both JME and JEE of the Jatonik poly-herbal mixture had low toxicity,profound MMPTpore induction,and enhanced ATPase activity,but an increased MDA production.Conclusion:Jatonik extracts may be a promising target for drug development in diseases where there is dysregulation of apoptosis,however,further studies are needed to better clarify the molecular mecha-nism involved in these phenomena.

外文关键词：

acute toxicityAgeratum conyzoides Linn.apoptosisHunteria umbellate(K.Schum.)Hallier f.Lepidium meyenii Walp.mitochondrial ATPasemitochondrial lipid peroxidationmitochondrial membrane permeabilitytransition porepolyherbal mixtureXylopia aethiopica

作者：

Olabinri P.Folashade、Ibrahim Damilare Boyenle、Tolulope A.Oyedeji、Fiyinfoluwa Demilade Ojeniyi、Adisa Ayobami Damilare、Leonard O.Ehigie、Adeola Folasade Ehigie

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作者单位：

Membrane Biochemistry and Biophysics Research Laboratory,Department of Biochemistry,Ladoke Akintola University of Technology,Ogbomoso 210214,Nigeria

College of Health Sciences,Crescent University Abeokuta,Abeokuta 111105,Nigeria

Department of Biochemistry,University of Lagos,Lagos 101017,Nigeria

Computational Biology/Drug Discovery Laboratory,Department of Biochemistry,Ladoke Akintola University of Technology,Ogbomoso 210214,Nigeria

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出版年：

2024

DOI：

10.1016/j.chmed.2023.06.002

中草药(英文版)