Abstract
Objective:Angelicae Sinensis Radix(ASR,Danggui in Chinese),Cistanches Herba(CH,Roucongrong in Chinese),Ginseng Radix et Rhizoma(PG,Renshen in Chinese),and Panacis Quinquefolii Radix(PQ.Xiyangshen in Chinese),widely used as medicine and dietary supplement around the world,are suscep-tible to fungal and mycotoxin contamination.In this study,we aim to analyze their fungal community by DNA metabarcoding.Methods:A total of 12 root samples were collected from three main production areas in China.The sam-ples were divided into four groups based on herb species,including ASR,CH,PG,and PQ groups.The fun-gal community on the surface of four root groups was investigated through DNA metabarcoding via targeting the internal transcribed spacer 2 region(ITS2).Results:All the 12 samples were detected with fungal contamination.Rhizopus(13.04％-74.03％),Aspergillus(1.76％-23.92％),and Fusarium(0.26％-15.27％)were the predominant genera.Ten important fungi were identified at the species level,including two potential toxigenic fungi(Penicillium citrinum and P.oxalicum)and eight human pathogenic fungi(Alternaria infectoria,Candida sake,Hyphopichia bur-tonii,Malassezia globosa,M.restricta,Rhizopus arrhizus,Rhodotorula mucilaginosa,and Ochroconis tsha-wytschae).Fungal community in ASR and CH groups was significantly different from other groups,while fungal community in PG and PQ groups was relatively similar.Conclusion:DNA metabarcoding revealed the fungal community in four important root herbs.This study provided an important reference for preventing root herbs against fungal and mycotoxin contamination.
基金项目
CAMS Innovation Fund for Medical Sciences(CIFMS)(2021-I2M-1-071)
NETL
NSTL
万方数据