首页|DNA metabarcoding analysis of fungal community on surface of four root herbs
DNA metabarcoding analysis of fungal community on surface of four root herbs
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NETL
NSTL
万方数据
Objective:Angelicae Sinensis Radix(ASR,Danggui in Chinese),Cistanches Herba(CH,Roucongrong in Chinese),Ginseng Radix et Rhizoma(PG,Renshen in Chinese),and Panacis Quinquefolii Radix(PQ.Xiyangshen in Chinese),widely used as medicine and dietary supplement around the world,are suscep-tible to fungal and mycotoxin contamination.In this study,we aim to analyze their fungal community by DNA metabarcoding.Methods:A total of 12 root samples were collected from three main production areas in China.The sam-ples were divided into four groups based on herb species,including ASR,CH,PG,and PQ groups.The fun-gal community on the surface of four root groups was investigated through DNA metabarcoding via targeting the internal transcribed spacer 2 region(ITS2).Results:All the 12 samples were detected with fungal contamination.Rhizopus(13.04％-74.03％),Aspergillus(1.76％-23.92％),and Fusarium(0.26％-15.27％)were the predominant genera.Ten important fungi were identified at the species level,including two potential toxigenic fungi(Penicillium citrinum and P.oxalicum)and eight human pathogenic fungi(Alternaria infectoria,Candida sake,Hyphopichia bur-tonii,Malassezia globosa,M.restricta,Rhizopus arrhizus,Rhodotorula mucilaginosa,and Ochroconis tsha-wytschae).Fungal community in ASR and CH groups was significantly different from other groups,while fungal community in PG and PQ groups was relatively similar.Conclusion:DNA metabarcoding revealed the fungal community in four important root herbs.This study provided an important reference for preventing root herbs against fungal and mycotoxin contamination.
NETL
NSTL
万方数据
