首页|Hypoglycemic activities of flowers of Xanthoceras sorbifolia and identification of anti-oxidant components by off-line UPLC-QTOF-MS/MS-free radical scavenging detection

Hypoglycemic activities of flowers of Xanthoceras sorbifolia and identification of anti-oxidant components by off-line UPLC-QTOF-MS/MS-free radical scavenging detection

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Objective:To identify phytochemical constituents present in the extract of flowers of Xanthoceras sorbifo-lia and evaluate their anti-oxidant and anti-hyperglycemic capacities.Methods:The AlCl3 colorimetric method and Prussian Blue assay were used to determine the contents of total flavonoids and total phenolic acids in extraction layers,and the bioactive layers was screened through anti-oxidative activity in vitro.The Waters ACQUITY UPLC system and a Waters ACQUITY UPLC BEH C18 column(2.0 mm × 150 mm,5 μm)were used to identify the ingredients.And anti-oxidative ingredients were screened by off-line UPLC-QTOF-MS/MS-free radical scavenging.The amelio-rative role of it was further evaluated in a high-fat,streptozotocin-induced type 2 diabetic rat model and the study was carried out on NADPH oxidase(PDB ID:2CDU)by molecular docking.Results:Combined with the results of activity screening in vitro,the anti-oxidative part was identified as the ethyl acetate layer.A total of 24 chemical constituents were identified by liquid chromatography-mass spectrometry in the ethyl acetate layer and 13 main anti-oxidative active constituents were prelim-inarily screened out through off-line UPLC-QTOF-MS/MS-free radical scavenging.In vivo experiments showed that flowers of X.sorbifolia could significantly reduce the blood glucose level of diabetic mice and alleviate liver cell damage.Based on the results of docking analysis related to the identified phyto-compounds and oxidase which involved in type 2 diabetes,quercetin 3-O-rutinoside,kaempferol-3-O-rhamnoside,isorhamnetin-3-O-glucoside,and isoquercitrin showed a better inhibitory profile.Conclusion:The ethyl acetate layer was rich in flavonoids and phenolic acids and had significant anti-oxidant activity,which could prevent hyperglycemia.This observed activity profile suggested X.sorbifolia flowers as a promising new source of tea to develop alternative natural anti-diabetic products with a high safety margin.

anti-hyperglycemic activityanti-oxidantflowers of Xanthoceras sorbifolia BungeNADPH oxidaseoff-line UPLC-QTOF-MS/MS-free radicalscavenging

Xiajing Xu、Yongli Guo、Menglin Chen、Ning Li、Yi Sun、Shumeng Ren、Jiao Xiao、Dongmei Wang、Xiaoqiu Liu、Yingni Pan

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School of Chinese Materia Medica,Key Laboratory for TCM Material Basis Study and Innovative Drug Development of Shenyang City,Shenyang Pharmaceutical University,Shenyang 110016,China

Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China

School of Pharmacy,Shenyang Pharmaceutical University,Shenyang 110016,China

Fourth National Investigation of Chinese materia medica resources in Liaoning ProvinceFourth National Investigation of Chinese materia medica resources in Liaoning ProvinceCareer Development Support Plan for Young and Middleaged Teachers in Shenyang Pharmaceutical UniversityKey Laboratory of Marine Biogenetic Resources,Ministry of Natural Resources

LN2018017LN2019019ZQN2021014HY202105

2024

10.1016/j.chmed.2022.11.009

中草药(英文版)
天津药物研究院 中国医学科学院药用植物研究所

中草药(英文版)

CSTPCD
影响因子：0.6
ISSN：1674-6384
年,卷(期)：2024.16(1)
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