首页|A new tetracyclic triterpenoid from endophytic fungus Fusarium sporotrichioides
A new tetracyclic triterpenoid from endophytic fungus Fusarium sporotrichioides
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Objective:To isolate bioactive compounds from the endophytic fungus Fusarium sporotrichioides isolated from Rauwolfia yunnanensis,and investigate their pharmacological activities.Methods:The chemical constituents were isolated and purified by combining with ODS column chro-matography,silica gel column chromatography and by performing semipreparative HPLC.Their struc-tures were established on the basis of 1D NMR(1H-NMR and 13C-NMR)and 2D NMR(1H-1H COSY,HSQC,HMBC and NOESY),as well as HRESIMS and comparison with literature data.In addition,the abso-lute configuration of compound 1 was determined by calculated ECD data.Results:One previously undescribed tetracyclic triterpenoid derivative,named as integracide L(1),12α-acetoxy-4,4-dimethyl-24-methylene-5α-cholesta-8,14-diene-2α,3β,1 1β-triol(2),12α-acetoxy-4,4-dime thyl-24-methylene-5α-cholesta-8-momoene-2α,3β,11 β-triol(3),12α-acetoxy-4,4-dimethyl-24-methy lene-5α-cholesta-8,14-diene-3β,11β-triol(4),and 12α-acetoxy-4,4-dimethyl-24-methylene-5α-cholesta-8-momoene-3β,11β-triol(5)were isolated from F.sporotrichioide.Moreover,compound 1 was rare tetracyclic triterpenoid with single methyl replacement at C-4 position.Conclusion:Compound 1 was a new tetracyclic triterpenoid isolated from the endophytic fungus F.sporotrichioides.In addition,compound 2 could inhibit the growth of three different human cancer cells significantly.Compounds 3 and 5 were found to possess better cytotoxic activities on HepG-2 cells than the other compounds,with IC50 values of(2.8±0.1)and(6.3±0.3)μmol/L respectively.
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