Abstract
Objective:To study secondary metabolites from endophytic fungus Colletotruchum sp.HK-08 originated from the leaves of Nerium indicum.Methods:The compounds were isolated by various column chromatographic techniques,and their struc-tures were elucidated by spectroscopic techniques[high resolution electrospray ionization mass spec-troscopy(HRESIMS),one-dimensional(1D)and two-dimensional(2D)nuclear magnetic resonance spectroscopy(NMR)],as well as comparison with literature data.The Ellman method was used to deter-mine the acetylcholinesterase(AChE)inhibitory activity.Results:Four indole derivatives were identified from Colletotruchum sp.HK-08,including 6'-hydroxy-monaspiloindole(1),2-(2-oxoindolin-3-yl)ethyl 2-(4-hydroxyphenyl)acetate(2),2-(2-oxoindolin-3-yl)ethyl 2-(2-hydroxyphenyl)acetate(3),and monaspiloindole(4).Compound 4 presented weak AChE inhi-bitory activity with IC50 value of(69.30±6.27)μmol/L[tacrine as the positive control,with IC50 value of(0.61±0.07)μmol/L].Conclusion:Compounds 1-3 were new compounds,and compound 4 had weak AChE inhibitory activity.
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