中草药(英文版)2024，Vol.16Issue(2) ：274-281.DOI:10.1016/j.chmed.2023.10.003

Huangqi Guizhi Wuwu Decoction suppresses inflammation and bone destruction in collagen-induced arthritis mice

Jiamin Bao Yongjia Song Minghui Hang Hao Xu Qiang Li Pengyu Wang Tao Chen Mengxiong Xia Qi Shi Yongjun Wang Xiaoyun Wang Qianqian Liang
中草药(英文版)2024，Vol.16Issue(2) ：274-281.DOI:10.1016/j.chmed.2023.10.003
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Huangqi Guizhi Wuwu Decoction suppresses inflammation and bone destruction in collagen-induced arthritis mice

Jiamin Bao 1Yongjia Song 2Minghui Hang 1Hao Xu 3Qiang Li 3Pengyu Wang 3Tao Chen 3Mengxiong Xia 3Qi Shi 1Yongjun Wang 1Xiaoyun Wang 4Qianqian Liang1
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作者信息

  • 1. School of Rehabilitation Science,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Spine Institute,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Key Laboratory of Theory and Therapy of Muscles and Bones,Ministry of Education,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China
  • 2. School of Rehabilitation Science,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China
  • 3. Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Spine Institute,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Key Laboratory of Theory and Therapy of Muscles and Bones,Ministry of Education,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China
  • 4. Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Spine Institute,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Key Laboratory of Theory and Therapy of Muscles and Bones,Ministry of Education,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shanghai Research Institute of Acupuncture and Meridian,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China
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Abstract

Objective:Rheumatoid arthritis(RA)is a chronic inflammatory and destructive arthritis,characterized by inflammatory infiltration and bone destruction.Huangqi Guizhi Wuwu Decoction(HGWD)is traditional Chinese medicine,which has been applied in the treatment of RA in clinical.The aim of this study was to investigate the therapeutic effect of HGWD on collagen-induced arthritis(CIA)mouse model.Methods:DBA/1J female mice were used to establish the collagen-induced arthritis(CIA)model.HGWD was administered intragastrically once a day for four weeks starting on the 22nd day after the first immu-nization.The body weight,hind paw thickness and clinical score were measured every five days.Gait analysis,histopathological staining,enzyme-linked immunosorbent assay(ELISA),ultrasound imaging and micro-computed tomography imaging were performed to determine the effects of HGWD treatment on inflammation and bone structure in this model.Moreover,Real-time PCR and Western blot analysis were used to detect inflammatory factors mRNA and protein levels after HGWD intervention in RAW 264.7 cells.Results:HGWD attenuated symptoms of arthritis,suppressed inflammatory synovium area and the serum levels of inflammatory factors,inhibited joint space enlargement in the knee and ankle joints,reduced numbers of osteoclasts,protected bone destruction,as well as improved motor function.HGWD decreased the expression of mRNA for inflammatory factors and the protein expression levels of p-NF-κB and IL-17.Conclusion:These results suggested that HGWD suppresses inflammation,attenuates bone erosion and maintains motor function in collagen-induced arthritis mice.

Key words

bone destruction/collagen-induced arthritis/Huangqi Guizhi Wuwu Decoction/inflammation/rheumatoid arthritis

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基金项目

国家自然科学基金(81822050)

国家自然科学基金(81920108032)

国家自然科学基金(81904227)

Shanghai"Science and Technology Innovation Action Plan"Medical Innovation Research Project(21Y11921400)

Program for Innovative Research Team of Ministry of Science and Technology of China(2015RA4002)

"Innovation Team"Development Projects(IRT1270)

Innovative Team Project of Scientific Research Project of Traditional Chinese Medicine of Shanghai Municipal Health Commission(2022CX001)

Shanghai TCM Medical Center of Chronic Disease(2022ZZ01009)

Jing'an District Health Research Project of Shanghai(2022MS03)

出版年

2024
中草药(英文版)
天津药物研究院 中国医学科学院药用植物研究所

中草药(英文版)

CSTPCD
影响因子：0.6
ISSN：1674-6384
参考文献量38
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