首页|Huangqi Guizhi Wuwu Decoction suppresses inflammation and bone destruction in collagen-induced arthritis mice

Huangqi Guizhi Wuwu Decoction suppresses inflammation and bone destruction in collagen-induced arthritis mice

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Objective:Rheumatoid arthritis(RA)is a chronic inflammatory and destructive arthritis,characterized by inflammatory infiltration and bone destruction.Huangqi Guizhi Wuwu Decoction(HGWD)is traditional Chinese medicine,which has been applied in the treatment of RA in clinical.The aim of this study was to investigate the therapeutic effect of HGWD on collagen-induced arthritis(CIA)mouse model.Methods:DBA/1J female mice were used to establish the collagen-induced arthritis(CIA)model.HGWD was administered intragastrically once a day for four weeks starting on the 22nd day after the first immu-nization.The body weight,hind paw thickness and clinical score were measured every five days.Gait analysis,histopathological staining,enzyme-linked immunosorbent assay(ELISA),ultrasound imaging and micro-computed tomography imaging were performed to determine the effects of HGWD treatment on inflammation and bone structure in this model.Moreover,Real-time PCR and Western blot analysis were used to detect inflammatory factors mRNA and protein levels after HGWD intervention in RAW 264.7 cells.Results:HGWD attenuated symptoms of arthritis,suppressed inflammatory synovium area and the serum levels of inflammatory factors,inhibited joint space enlargement in the knee and ankle joints,reduced numbers of osteoclasts,protected bone destruction,as well as improved motor function.HGWD decreased the expression of mRNA for inflammatory factors and the protein expression levels of p-NF-κB and IL-17.Conclusion:These results suggested that HGWD suppresses inflammation,attenuates bone erosion and maintains motor function in collagen-induced arthritis mice.

bone destructioncollagen-induced arthritisHuangqi Guizhi Wuwu Decoctioninflammationrheumatoid arthritis

Jiamin Bao、Yongjia Song、Minghui Hang、Hao Xu、Qiang Li、Pengyu Wang、Tao Chen、Mengxiong Xia、Qi Shi、Yongjun Wang、Xiaoyun Wang、Qianqian Liang

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School of Rehabilitation Science,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China

Longhua Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China

Spine Institute,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China

Key Laboratory of Theory and Therapy of Muscles and Bones,Ministry of Education,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China

Shanghai Research Institute of Acupuncture and Meridian,Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China

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国家自然科学基金国家自然科学基金国家自然科学基金Shanghai"Science and Technology Innovation Action Plan"Medical Innovation Research ProjectProgram for Innovative Research Team of Ministry of Science and Technology of China"Innovation Team"Development ProjectsInnovative Team Project of Scientific Research Project of Traditional Chinese Medicine of Shanghai Municipal Health CommissionShanghai TCM Medical Center of Chronic DiseaseJing'an District Health Research Project of Shanghai

81822050819201080328190422721Y119214002015RA4002IRT12702022CX0012022ZZ010092022MS03

2024

10.1016/j.chmed.2023.10.003

中草药(英文版)
天津药物研究院 中国医学科学院药用植物研究所

中草药(英文版)

CSTPCD
影响因子：0.6
ISSN：1674-6384
年,卷(期)：2024.16(2)
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