首页|Integrative analysis of metabolome and transcriptome provides new insights into functional components of Lilii Bulbus

Integrative analysis of metabolome and transcriptome provides new insights into functional components of Lilii Bulbus

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Objective:Lilium brownii var.viridulum(LB)and L.lancifolium(LL)are the main sources of medicinal lily(Lilii Bulbus,Baihe in Chinese)in China.However,the functional components of these two species respon-sible for the treatment efficacy are yet not clear.In order to explore the therapeutic material basis of Lilii Bulbus,we selected L.davidii var.willmottiae(LD)only used for food as the control group to analyze the differences between LD and the other two(LB and LL).Methods:Metabolome and transcriptome were carried out to investigate the differences of active com-ponents in LD vs LB and LD vs LL.Data of metabolome and transcriptome was analysed using various analysis methods,such as principal component analysis(PCA),hierarchical cluster analysis(HCA),and so on.Differentially expressed genes(DEGs)were enriched through KEGG and GO enrichment analysis.Results:The PCA and HCA of the metabolome indicated the metabolites were clearly separated and varied greatly in LL and LB contrasted with LD.There were 318 significantly differential metabolites(SDMs)in LD vs LB group and 298 SDMs in LD vs LL group.Compared with LD group,the significant up-regulation of steroidal saponins and steroidal alkaloids were detected both in LB and LL groups,especially in LB group.The HCA of transcriptome indicated that there was significant difference in LB vs LD group,while the dif-ference between LL and LD varied slightly.Additionally,47 540 DEGs in LD vs LB group and 18 958 DEGs in LD vs LL group were identified.Notably,CYP450s involving in the biosynthesis of steroidal saponins and steroidal alkaloids were detected,and comparing with LD,CYP724,CYP710A,and CYP734A1 in LB and CYP90B in LL were all up-regulated.Conclusion:This study suggested that steroidal saponins and steroidal alkaloids maybe the representa-tive functional components of Lilii Bulbus,which can provide new insights for Lilii Bulbus used in the research and development of classic famous formula.

Lilium brownii F.E.Brown var.viridulumBakerLilium davidii var.willmottiae(E.H.Wilson)RaffillLilium lancifolium Thunb.metabolometranscriptome

Wenjun Wei、Tao Guo、Wenguang Fan、Mengshan Ji、Yu Fu、Conglong Lian、Suiqing Chen、Wenjing Ma、Wenfang Ma、Shuying Feng

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School of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China

Henan Engineering Research Center of Medicinal and Edible Chinese Medicine Technology,Zhengzhou 450046,China

School of Life Science and Engineering,Lanzhou University of Technology,Lanzhou 730050,China

Lanzhou Shibai Agricultural Biotechnology Co.,Ltd.,Lanzhou 730050,China

Medical College,Henan University of Chinese Medicine,Zhengzhou 450046,China

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2022 Provincial Science and Technology Research and Development Plan United FundHenan Provincial High-Level Talents International Training Funding Project

2223014200752021-72

2024

10.1016/j.chmed.2023.10.004

中草药(英文版)
天津药物研究院 中国医学科学院药用植物研究所

中草药(英文版)

CSTPCD
影响因子：0.6
ISSN：1674-6384
年,卷(期)：2024.16(3)