Identification and characterization of chemical constituents in Mahuang Guizhi Decoction and their metabolites in rat plasma and brain by UPLC-Q-TOF/MS

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外文摘要：Objective:Mahuang Guizhi Decoction(MGD),an essential herbal pair in traditional Chinese medicine,is able to release cold,fever and asthma,mainly containing alkaloids,flavonoids,phenylpropanoids and amino acids.However,the absorption and distribution of these four category compounds in vivo still remained unclearly.Methods:In our research,we utilized UPLC-Q-TOF-MS technique to identify the constituents within MGD,as well as the prototypes of MGD and their metabolites absorbed in plasma and brain.We further profiled the drug-time curve of prototypes and metabolites of MGD both in plasma and brain.Results:Our results showed that 105 constituents were characterized in MGD.Thirty of them could be absorbed into blood,and ten of them could be distributed into brain.We also discovered eight new bio-transformed metabolites in blood,and a half of which could pass through the blood-brain barrier.In addition,all components detected in vivo could be absorbed and distributed immediately.Conclusion:These findings provide an approachable method to analyze the potential bio-active com-pounds in MGD and their in vivo behaviors,which could promote the efficacious material basis study of MGD and the security of clinical utilization.

外文关键词：

chemical constituentsMahuag Guizhi Decoctionmetabolitesrat plasma and brainUPLC-Q-TOF-MS

作者：

Ping Wei、Shiying Huang、Juan Yang、Mo Zhao、Qiugu Chen、Xiaoyu Deng、Jianping Chen、Yisheng Li

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作者单位：

Pingshan District Maternal & Child Healthcare Hospital of Shenzhen,Pingshan General Hospital of Southern Medical University,Shenzhen 518118,China

Shenzhen Key Laboratory of Hospital Chinese Medicine Preparation,Shenzhen Traditional Chinese Medicine Hospital,The Fourth Clinical Medical College of Guangzhou University of Chinese Medicine,Shenzhen 518000,China

Shenzhen Longgang Otorhinolaryngology Hospital,Shenzhen 518100,China

基金：

Shenzhen Science and Technology ProgramShenzhen Science and Technology ProgramShenzhen Science and Technology ProgramFoundation of Guangdong Provincial Key Laboratory of Functional Substances in Medicinal Edible Resources and Healthcare ProductsSanming Project of Medicine in Shenzhen

项目编号：

JCYJ20210324140204011JSGG20210802093208023ZDSYS201606081515458GPKLFSHP202101SZZYSM202111002

出版年：

2024

DOI：

10.1016/j.chmed.2024.01.006

中草药(英文版)

天津药物研究院中国医学科学院药用植物研究所

中草药(英文版)

CSTPCD

影响因子：0.6

ISSN：1674-6384

年,卷(期)：2024.16(3)