Objecl To isolate and identify the chemical constituents of Pogostemon cablin (Blanco)Benth. For the purpose to screen new natural antifungal drugs. Methods The compounds were isolated by chromatography. Their chemical structures were elucidated with UV, IR, MS, NMR (1H, 3C, DEPT) spectroscopy. The antifungal activities of the four isolated compounds were examined in vitro by media dilution method. Results With antifungal guided fractionation, eight flavonoids were isolated from the alcoholic extract of the whole plant. Their structures were elucidated as 5-hydroxy-7, 3′, 4′-trimethoxyflavanone ( Ⅰ ), 5-hydro,xy-7, 4′-dimethoxyflavanone ( Ⅱ ), 3, 5,-dihydroxy-7, 4′-dimethoxyflavone ( Ⅲ ),5-hydroxy-3, 7, 4′-trimethoxyflavone (Ⅳ), 5-hydroxy-3, 7, 3′, 4′-tetramethoxyflavone (Ⅴ), 5, 4′-di-hydroxy-3, 7, 3′-trimethoxyflavone (Ⅵ), 5, 4′-dihydroxy-7-ethoxyflavone (Ⅶ), and 3, 5, 7, 3′, 4′-pentahydroxyflavone (Ⅷ) on the basis of spectroscopic data. The antifungal activities of compounds Ⅰ ,Ⅲ , Ⅴ , Ⅵl were exanined in vitro. Conclusion All the flavonoids, except Ⅴ and Ⅵ were isolated from this plant for the first time. The four examined compounds exhibited certain antifungal activities against six strains of fungi in vitro.
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