Objective To study the chemical constituents from Pseudolaricis Cortex with antifungal activities. Methods The crude powder of Pseudolaricis Cortex was refluxed with 95% ethanol and different fractions were obtained with different solvants. In vitro antifungal results revealed that EtOAc fraction showed inhibitory effects on Candida albicans. Compounds were isolated by column chromatography on silica gel, Sephadex LH-20, and preparative HPLC from EtOAc fraction of Pseudolaricis Cortex and their structures were elucidated mainly by spectral data. Results Five compounds were identified as pseudolaric acid B (1), pseudolaric acid C (2), vanillic acid (3), vanillic acid-4-O-β-D-allopyranoside (4), and 17-hydroxypseudolaric acid B (5), respectively. Conclusion Compound 5 is a new compound named pseudolaric acid I, and compound 4 is isolated from this plant for the first time. Compound 1 shows stronger activity against the growth of Candida albicans, while compounds 2 and 5 have weak antifungal activities in vitro.
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