Induction, proliferation, and differentiation of Bletilla striata callus
Objective To establish the regeneration system of Bletilla striata callus for induction, proliferation, and differentiation. Methods MS was selected as the basic medium. To study the effective factors on induction, proliferation, and differentiation of callus of B. striata with different explants and to optimize the plant growth regulators, kinds, and concentration ratio of plant growth regulators by orthogonal test design. Results The optimal explants were false bulbs of B. striata. MS medium added with 1.0 mg/L 6-benzylaminopurine (6-BA) and 2.0 mg/L 2, 4-dichlorophenoxy acetic acid (2, 4-D), not only could induce callus formation, but also benefit to the differentiation of callus. MS medium added with 1.0 mg/L 6-BA and 3.0 mg/L 2,4-D promoted the proliferation of callus, and the proliferation coefficient was 7.8. Adding with 0.5 mg/L thidiazuron (TDZ) to the differentiation medium improved the differentiation rate of callus. Conclusion MS + 6-BA 1.0 mg/L + 2, 4-D 2.0 mg/L is the optimal medium for the induction of callus; MS + 6-BA 1.0 mg/L + 2, 4-D 3.0 mg/L is the optimal medium for the proliferation of callus; MS + 6-BA 1.0 mg/L + 2, 4-D 2.0 mg/L + TDZ 0.5 mg/L is the optimal medium for the differentiation of callus.
NETL
NSTL
万方数据
维普
