Determination of seven components in Forsythia suspensa by RP-HPLC
Objective To establish an HPLC method for the simultaneous determination ofcafferic acid, forsythoside A, forsythoside B, rutin, hyperoside, forsythin, and arctigenin in Forsythia suspensa.Methods The analysis was carried out on an Inertsil ODS-3 C18 column (250 mm × 4.6 mm, 5 μm).The mobile phase was composed of acetonitrile and 0.2％ phosphorie acid aqueous with gradient elution.The detection wavelength was set at 275 um.The flow rate was 1.0 mL/min at column temperature of 30 ℃.Results Cafferic acid, forsythoside A, forsythoside B, rutin, hyperoside, forsythin, and arctigenin were well separated by this method, and showed a good linearity in the ranges of 18.24-91.20, 5.88-29.40, 132.60-663.00, 8.34-41.70, 1.96-9.80, 7.60-38.00, and 11.34-56.70 μg/mL, respectively.The average recoveries of the seven components were 97.7％, 96.7％, 102.6％, 101.3％, 93.2％, 91.8％, and 96.7％ and the RSD values were 2.3％, 1.4％, 2.4％, 2.2％, 1.0％, 1.0％, and 1.3％, respectively.Conclusion The established method is accurate, reliable, and could be used for the simultaneous determination of the seven components in F..suspense, which provides a scientific basis for the quality evaluation ofF.suspense.
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维普
