Optimization of tissue culture conditions for Pfaffia paniculata
Objective To establish and optimize the technique of tissue culture and rapid propagation of Pfaffia paniculata.Methods Using MS and 1/2MS basic media,the effects of various combinations of plant growth regulators (6-BA,NAA,IBA,and IAA) on the seedlings subculture and rooting culture were studied by orthogonal design.Results The effective medium for cluster buds-inducing and subculture was MS + 6-BA 1.5 mg/L + NAA 0.1 mg/L + IBA 0.2 mg/L,and the propagation coefficient was over 6.0 per 30 d; The best rooting medium was 1/2 MS + NAA 1.0 mg/L + IBA 0.2 mg/L,and the rooting rate was 100％.The rooting seedlings were transplanted in well-drained sand bed and the survival rate was 98％.Conclusion The method in the experiment could provide a basis on protecting the mild resources ofP.paniculata exploring the artificial resources,and discussing the new way breedings.
NETL
NSTL
万方数据
维普
