Objective To study the genetic diversity of Dipsacus asper from different populations and provide a reference for the rational utilization of its germplasm.Methods The genetic diversity of the 14 populations of D.asper was analyzed by SRAP molecular markers.Results Ten pairs of primers produced 124 sites,among which 102 were polymorphic sites.The percentage of polymorphic loci (PPL) was 82.26％.The Nei's genetic diversity index (H) and the Shannon's information index (Ⅰ) were 0.280 0 and 0.435 3,respectively.At the population level,PPL was 53.92％,H was 0.121 2-0.244 0,and I was 0.179 6-0.361 1.The genetic diversity values of the five populations were relatively high,and the populations had the characteristics of high altitude and microhabitat.Genetic differentiation coefficient (Gst) was 0.293 0,gene flow (Nm) was 1.206 4.Cluster analysis based on genetic similarity indicated that the 14 populations could be divided into three groups.Conclusion The genetic diversity among the populations ofD.asper was at relatively high level.The genetic variance ofD.asper mainly existed within the populations.The high genetic diversity could be attributed to the geographical position (altitude) and climate,while geographic isolation (microhabitat) was another important factor for the genetic variance within the populations.
Dipsacus asper Wall.ex Henrygermplasm resourcesgenetic diversitypopulationsequence related amplified polymorphism
NETL
NSTL
维普
万方数据
