Effects of exogenous nitric oxide on seed germination and seedling physiological characteristics of Isatis indigotica under NaCl stress
Objective To offer the reference and method for salt damage in the cultivation of Isatis indigotica and to study the effects of exogenous nitric oxide (NO) on the seed germination and early seedling growth of I.indigotica under NaCl stress.Methods Hoagland's nutrient solution was used to culture the seeds,and the changes of germination energy,germination percentage,germination index,vigor index,germination velocity index,mean germination time,seedling height,root length,seedling weight,and root weight ofI.indigotica,and the changes of the MDA content,the activities of SOD and POD ofI.indigotica leaves after the treatment with exogenous NO donor,sodium nitroprusside (SNP) at different concentration under 60 mmol/L NaCl stress were determined.Results Under 60 mmol/L NaCl stress,the seed germination and early seedling growth of I.indigotica were obviously inhibited,which were increased after 0.01-0.5 mmol/L SNP treatment and the maximum index was obtained under 0.1 mmol/L SNP treatment,while the seedling growth was markedly reduced after 1.0 mmol/L SNP treatment.Compared with 60 mmol/L NaCl treatment,the germination percentage and germination energy after 0.1 mmol/L SNP treatment were increased by 12.91％ and 63.90％; The germination index and vigor index were increased by 16.71％ and 44.77％; The germination velocity index was increased by 23.64％; The mean germination time was shortened by about 0.1 d; The seedling height,root length,seedling weight,and root weight were increased by 43.61％,35.05％,51.79％,and 82.14％; The MDA content was decreased by 34.83％; The activities of SOD and POD were increased by 43.78％ and 39.85％,respectively.Conclusion SNP (0.1 mmol/L) could greatly alleviate the NaCl stress for the seed germination and early seedling growth of I.indigotica,enhance the anti-oxidative capacity of leaves,and improve the salt resistance ability of the seed and seedling.But the SNP treatment at high concentration (1.0 mmol/L) could seriously damage the seedling of I.indigotica under NaCl stress.
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万方数据
维普
