摘要
目的 研究小花清风藤Sabia parviflora茎叶的化学成分.方法 利用硅胶、Sephadex LH-20、反相硅胶、MCI等柱色谱以及半制备高效液相色谱等多种色谱技术进行分离纯化,运用NMR、MS等波谱学方法以及结合文献数据鉴定化合物结构.结果 从小花清风藤茎叶的石油醚萃取部位分离得到14个化合物,包括7个五环三萜类化合物,4个生物碱类化合物,3个其他类化合物;分别鉴定为β-谷甾醇(1)、木栓酮(2)、(20S)-3-oxo-20-hydroxytaraxastane (3)、9-芴酮(4)、N-formyldehydroanonain (5)、桦木酸(6)、dehydroformouregine (7)、棕榈酸(8)、20-hydroxy-lupan-3-one (9)、3-氧代齐墩果酸(10)、古柯三萜二醇(11)、桦木酸甲酯(12)、N-formyl-annonain(Z)(13)、N-formyl-O-methylisopiline (14).结论 化合物3～10、12～14为首次从该植物以及该属植物中得到,首次报道了化合物5、7、14的13C-NMR谱归属.
Abstract
Objective To investigate the chemical constituents from the stems and leaves of Sabia parviflora.Methods Column chromatographies over silica gel,Sephadex LH-20,reverse phase C18,MCI,and semi-preparative HPLC were used repeatedly for separation and purification of chemical constituents,and their structures were identified by NMR and MS spectra data with those reported in literature.Results Fourteen compounds were obtained from the petroleum ether extract ofS.parviflora,and identified as β-sitosterol (1),mominine (2),(20S)-3-oxo-20-hydroxytaraxastane (3),fluoren-9-one (4),N-formyldehydroanonain (5),betulinic acid (6),dehydroformouregine (7),palmitic acid (8),20-hydroxy-lupan-3-one (9),3-oxooleanolic acid (10),erythrodiol (11),methyl betulinate (12),N-formyl-annonain (Z) (13),and N-formyl-O-methylisopiline (14).Conclusion Among the 14 compounds,there are seven pentacyclic triterpenes,four alkaloids,and three other compounds;compounds 3-10 and 12-14 are isolated from this plant and the plants ofSabia Colebr.for the first time,and the 13C-NMR spectral assignments of compounds 5,7,and 14 are reported for the first time.
基金项目
贵州省中药现代化科技产业研究开发专项项目(黔科合SY字[2014]3081-1号)
国家科技期刊平台
NSTL
维普
万方数据