HPLC method for simultaneous determination of nine components from leaves of Rheum officinale by HPLC
Objective To establish an HPLC method for determing nine components from the leaves of Rheum officinale,such as gallic acid,senna glycosides B,rhubarb phenol-1-O-glucoside,emodin-8-O-glucoside,aloe-emodin,rhein,emodin,rhubarb,and emodin methyl ether,as well as to explore the scientific evidence for reasonable exploitation and utilization of medicinal rhubarb leaf.Methods Takeing C18 chromatographic column (250 mm × 4.6 mm,5 microns),methanol-0.2％ acetic acid water as mobile phase,gradient elution and flow rate of 1 mL/min,column temperature 30 ℃,and detection wavelength of 260 nm.Results Gallic acid,senna glycosides B,rhubarb phenol-1-O-glucoside,emodin-8-O-glucoside,aloe-emodin,rhein,emodin,rhubarb,and emodin methyl ether had the good linear relationship,respectively in the range of 20.2-606.0,79.3-2 379.0,10.1-301.8,14.8-443.7,0.7-2.2,0.13-3.9,12.4-372.0,38.8-1 164.0,6.2-185.4 ng (r＞ 0.999 6);The nine kinds of the ingredients of the average recovery were 95.76％-95.76％,RSD was 1.46％-2.43％.Conclusion This method is simple and accurate,and with good effect and reliable results for the separation and determination of the components from the leaves ofR.officinale,which can provide the reference for its rational development and utilization.
leaves of Rheum officinalelHPLCgallic acidsenna glycosides Brhubarb phenol-1-O-glucosideemodin-8-O-glucosidealoe-emodinrheinemodinrhubarbemodin methyl ether
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