ISSR analysis and identification of medicinal plants Rosa chinensis, Rosa rugosa,and their relative species
Objective To study the genetic diversity and genetic relationship of medicinal plants Rosa chinensis,R.rugosa,and their relative species by ISSR molecular marker technique,and provide the reference for Rosa L.germplasm identification and breeding.Methods Fifteen species (including 33 samples) of medicinal herbs R.chinensis,R.rugosa,and their relative species were studied by ISSR-PCR markers.Nei's genetic diversity index (H) and other parameters of genetic information were calculated by POPGEN 32,and a cluster dendrogram of different samples was established based on the unweighted pair-group method with arithmetic mean (UPGMA) by NTSYS-pc software.Results Six ISSR primers generated 110 loci of which 109 loci were polymorphic.The average percentage of polymorphie bands (PPB) was 99.09％.Nei's genetic diversity index (H) and Shannon's information index (I) were 0.370 5 and 0.546 4,and the coefficient of genetic differentiation (Gst) and gene flow (Nm) were 0.886 8 and 0.063 8 between the species levels.The genetic distance (D) varied from 0.169 1 to 0.730 2.In the cluster dendrogram,15 species were clustered into six groups at the level of Genetic similarity coefficient (GS) 0.60.Conclusion The results of ISSR analysis reveal that medicinal plants R.chinensis,R.rugosa and their relative species had the plentiful genetic diversity and the genetic relationships were consistent with morphological characters of taxonomy.ISSR method is efficient for identification of R.chinensis,R.rugosa,and their relative species,which could provide a scientific basis for the resource collection and identification of the species in Rosa L.
Rosa chinensis Jacq.Rosa rugosa Thunb.ISSRgenetic diversityidentification
NETL
NSTL
万方数据
维普
