Objective To investigatethe chemical constituents from Paris thibetica and their antitumor activities.Methods The separations and purifications were taken by column chromatography over silica gel,Sephadex LH-20,ODS-C18,and semi-preparative HPLC.The in vitro antitumor activities of the isolated compounds were studied by MTT method.Results Fourteen compounds were isolated from ethanol extract of the rhizomes ofP thibetica,which were identified as paris saponin Ⅴ (1),paris saponin Ⅰ (2),paris saponin Ⅱ (3),paris saponin Ⅵ (4),pennogennin-3-O-α-L-arabinofuranosyl-(1→4)-β-D-glucopyranoside (5),paris saponin H (6),pennogennin-3-O-α-L-rhamnopyranosyl-(1→4)-[α-L-rhamnopyranosyl-(1→2)]-β-D-glucopyranoside (7),paris saponin Ⅶ (8),parisyunnanoside G (9),trikamsteroside E (10),stigmasterol-3-O-β-D-glucopyranoside (11),β-sitosteryl palmitate (12),β-amyrin (13),and 4-hydroxy-5-methylfuran-3-carboxylic acid (14).Conclusion All compounds are furstly reported from P.thibetica,and this is the first report of compounds 10 and 12-14 from the genus Paris L.Compounds 1-8 show cytotoxicities against BEL-7402.Among the tested compounds,compound 3 exhibits the strongest cytotoxicity with IC50 value of 0.48 μmol/L.
Paris thibetica Franchsteroidal saponinsparis saponin Ⅰparis saponin Ⅱantitumor activities
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