Identification of DNA barcoding of common medicinal plants in Lamiaceae
Objective To screen a proper DNA barcoding suitable for common medicinal plants in Lamiaceae.Methods The rDNA ITS sequence firom 33 kinds of common medicinal plants in Lamiaceae and the chloroplast matK gene sequence from 40 kinds of common medicinal plants in Lamiaceae were amplified by PCR and sequenced.The interspecific and intraspecific Kimura 2-parameter distance (K2P) and the mutation sites in each sequence were calculated,and barcoding gap of sequence was evaluated by MEGA6.0.The phylogenetic trees were constructed on the basis of Neighbor-Joining method.Results The total length of ITS sequence was 620-698 bp,the average G + C content was 62.8％,the chloroplast matK gene sequence length was 859932 bp,the average G + C content was 34％,and the ITS sequence and matK gene sequence have obvious DNA barcoding gap,but the matK DNA barcoding gap was less than that of ITS gene sequence.From clustering analysis,the matK gene can identify the different species in Lamiaceae better than ITS sequence.Conclusion matK sequence can be used as a preferred sequence to identify the species in Lamiaceae.
NETL
NSTL
万方数据
维普
