Objective To isolate anti-renin constituents from Trichosanthis Pericarpium based on bioactivity tracking separation.Methods T.Pericarpium was extracted with boiling water and precipitated by ethanol,the supernate was collected and dialyzed,and the retentate was lyophilized giving GLP,then successively separated by DEAE Sepharose Fast Flow anion-exchange and Superdex-75 gel permeation chromatographic steps,to give GLP-5-1.A combination of HPGPC,monosaccharide composition,and anti-renin studies was performed to investigate the structure and bioactivity.Results An anti-renin polysaccharide,GLP-5-1,was obtained from T.Pericarpium based on activity tracking,the average molecular weight was estimated to 3 722,monosaccharide composition analysis showed GLP-5-1 contained arabinose,manmose,glucose,and galactose with the ratio of 5.5 ∶ 4.4 ∶ 6.9 ∶ 10.0.GLP-5-1 showed potent anti-renin effect with the IC50 was (87.4 ± 6.8) μg/mL.Conclusion In this study,a low molecular weight polysaccharide with anti-renin effect was isolated from T.Pericarpium,which could lay the foundation for the substance basis study of T.Pericarpium.
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维普
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