摘要
目的 研究半边旗Pteris semipinnata的化学成分.方法 综合应用硅胶、Sephadex LH-20凝胶、ODS、大孔树脂、MCI Gel柱色谱和半制备高效液相色谱法分离纯化,并通过NMR和HR-ESI-MS法鉴定结构.结果 从半边旗全草甲醇提取物中共分离20个化合物,分别鉴定为芹菜素(1)、芹菜素-7-O-β-D-吡喃葡萄糖苷(2)、芹菜素-7-O-β-D-龙胆二糖苷(3)、芹菜素-7-O-β-D-吡喃葡萄糖-4'-O-α-L-吡喃鼠李糖苷(4)、异佛莱心苷(5)、木犀草素(6)、木犀草苷(7)、木犀草素-7-O-β-D-龙胆二糖苷(8)、槲皮素-3-O-β-D-吡喃葡萄糖苷(9)、山柰酚-3-O-β-D-吡喃葡萄糖苷(10)、芦丁(11)、表没食子儿茶素(12)、松脂素-4-O-β-D-吡喃葡萄糖苷(13)、连翘苷(14)、岩白菜素(1S)、原儿茶酸(16)、反式咖啡酸(17)、没食子酸(18)、β-谷甾醇(19)、β-胡萝卜苷(20).结论 化合物3、5、12、13、15～18为首次从该属植物中分离得到,化合物2～5、8～13、15～20为首次从该植物中分离得到.
Abstract
Objective To study the chemical constituents from the whole plants of Pteris semipinnata.Methods The chemical constituents were isolated by silica gel,sephadex LH-20,ODS,macroporous resin,MCI Gel resin and semi-preparation HPLC.Their structures were determined on the basis of NMR and MS analysis.Results Twenty compounds were isolated and identified as apigenin (1),apigenin-7-O-β-D-glucopyranoside (2),apigenin-7-O-β-D-gentiobioside (3),apigenin-7-O-β-D-glucopyranosyl-4'-O-α-L-rhamnopyranoside (4),isoviolanthin (5),luteolin (6),luteoloside (7),luteolin-7-O-β-D-gentiobioside (8),quercetin-3-O-β-D-glucopyranoside (9),kaempferol-3-O-β-D-glucopyranoside (10),rutin (11),epigallocatechin (12),pinoresinol-4-O-β-D-glucopyranoside (13),phillyrin (14),bergeninum (15),protocatechuic acid (16),trans-caffeic acid (17),gallic acid (18),β-sitosterol (19),and β-daucosterol (20).Conclusion Compounds 3,5,12,13,and 15-18 are isolated from the plants of Pteris,and 2-5,8-13,and 15-20 are isolated from this plant for the first time.
基金项目
广东省高等学校优秀青年教师培养计划(Yq2013045)
国家科技期刊平台
NSTL
维普
万方数据