大黄甘草汤胶囊剂急性毒性及药效学实验研究
Study on efficacy and acute toxicology of Dahuang Gancao Decoction Capsule
赵琳琳 1李盖 2赵媛 1李唐棣 1刘春艳1
作者信息
- 1. 华北理工大学药学院,河北唐山063000
- 2. 华北理工大学附属医院,河北唐山063000
- 折叠
摘要
目的 考察大黄甘草汤胶囊剂的药效及安全性,为临床应用奠定基础.方法 最大给药量法考察大黄甘草汤胶囊剂的急性毒性,昆明小鼠1d内分别ig给予1、2、3次最大剂量(0.2 g/mL,25 mL/kg)的大黄甘草汤胶囊剂,即低、中、高剂量组(n=10).7d后,通过测定血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平,计算肝脏、肾脏指数,进行肝组织HE染色观察,评价其急性毒性.炭末推进实验法评价大黄甘草汤促进胃肠道蠕动效应.结果 与对照组比较,大黄甘草汤胶囊剂低、中、高剂量组的肝脏指数和肾脏指数均没有明显差异(P＞0.05);小鼠血清中ALT、AST没有明显变化(P＞0.05);肝组织病理切片未见异常现象.炭末推进实验,给药中剂量组小鼠小肠的推进率为(77.54±4.29)％,显著高于对照组[(64.74±5.17)％,P＜0.01].结论 制备的大黄甘草汤胶囊剂,短期内服用肝脏无损伤,具有较高的安全性;且该制剂有明显的泻下作用,能够有效地促进小鼠胃肠道的蠕动.
Abstract
Objective To ensure the efficacy and safety ofDahuang Gancao Decoction Capsule (DGDC) through experimentation on animals.Methods Using matched control of maximum dosage to identify acute toxicity of the capsule.The maximum dosage of Kunming mouse gavage was used once a day for low-dose group,twice a day for mid-dose group,and three times a day for high-dose group (n =10).After 7 d,diagnose toxicity of DGDC was measured serumliver enzyme,calculated liver index and kidney index,and observing liver HE staining.It was diagnosed incharcoal driving groups that how much the DGDC promotes gastrointestinal peristalsis.Results Liver index and kidney index in low-,mid-,and high-dose groups was calculated the same compared with no-dosage group,which means no statistical significance (P ＞ 0.05).Meantime,ALT and AST in the mice's serumdose not change obviously,which means no statistical significance (P ＞ 0.05).Abnormities were not found in pathological section of hepatictissue.small intestine propelling rates incharcoal driving groups were (77.54 ± 4.29)％,which was much higher than theno-driving groups [(64.74 ± 5.17)％,P ＜ 0.01].Conclusion The capsule has no harm to liver in a certain time of oral use.It is effective in causing diarrhea,and can promote gastrointestinal peristalsis in the mouse.
关键词
大黄甘草汤/急性毒性/肝毒性/胃肠道蠕动/炭末推进实验Key words
Dahuang Gancao Decoction/acute toxicity/hepatotoxicity/gastrointestinal peristalsis/carbon medicinalis drive experiment引用本文复制引用
出版年
2018
国家科技期刊平台
NSTL
维普
万方数据