Objective To study the chemical constituents and anti-inflammatory activities of medicinal insect Vespa velutina auraria.Methods The compounds were isolated and purified by silica gel,Sephadex LH-20 and semi-preparative liquid chromatography,while the structures of compounds were characterized and analyzed by nuclear magnetic resonance(NMR)technique.The mouse macrophage RAW264.7 inflammation model was induced by lipopolysaccharide(LPS)combined with interferon γ(IFN-γ),the effects of extracts and monomers on the mRNA expression of inflammatory factors were investigated.Results A total of 16 compounds were isolated from ethyl acetate fractions and identified as catechol hydroquinone(1),4-hydroxybenzaldehyde(2),methyl 3-(4-hydroxyphenyl)propionate(3),4-hydroxyphenethy acetate(4),3,4-dihydroxy-phenethyl alcohol(5),3,4-dihydroxyphenyl ethanol ketone(6),3,4-dihydroxy-benzoic acid(7),divesamides A(8),methyl-2-(2-hydroxy-propionyloxy)propionate(9),4-methoxybenzyl alcohol(10),3,4-dihydroxy-benzoic methyl ester(11),α-ethyl glucoside(12),papuline(13),N-acetyl-dopamine(14),2-(4-methylphenyl)ethanol(15),thymine(16).The alcohol extracts,ethyl acetate fractions,n-butanol fractions and water fractions of V.velutina auraria could effectively reduce the relative expression of iNOS gene of cellular inflammatory factor in RAW264.7 induced by LPS+IFN-γ when the administration concentration of ethyl acetate,n-butanol and water fractions was 300 μg/mL.Compounds 2,3,5,6,9-11 inhibited the expression of TNF-α mRNA in RAW264.7 cells induced by LPS combined with IFN-γ(P＜0.01).Conclusion The alcoholic extracts and different polar parts of V.velutina auraria can reduce the expression of iNOS in inflammatory cells and have some anti-inflammatory effects.Compounds 1-16 are isolated and identified from the ethyl acetate portion of V.velutina auraria for the first time,of which 2,3,5,6,9-11 may be potential active monomers.
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