Transcriptome characteristic and discovery of related genes involved in active ingredients biosynthesis of Sarcandra glabra leaves
Objective To explore the molecular basis of active ingredients biosynthesis in the leaves of Sarcandra glabra,and exploit this resource reasonably.Methods S.glabra leaf was analyzed by transcriptome sequencing with the BGISEQ-500 platform.After filtration and the Trinity assembly,the unigenes were analyzed by bioinformatics.Results A total of 75 862 unigenes with an average length of 980 bp were obtained.Among them,42 369 unigenes,accounting for 55.85%of the total,were successfully annotated in seven public databases.A total of 21 801 unigenes were categorized into 55 GO terms among three categories:biological process,cellular component and molecular function.KEGG enrichment analyses shown that there were two pathways,namely"metabolism of terpenoids and polyketides"and"biosynthesis of other secondary metabolites",may be closely related to the formation of pharmacodynamic basis.Moreover,it was discovered that 47 unigenes,14 unigenes and 62 unigenes might participate in terpenoid backbone,sesquiterpenoids and flavonoid biosynthesis,respectively.In addition,a total of 19 690 SSR sites were detected in the transcriptome sequences,of which the highest proportion of di-nucleotides was 46.63%.A total of 11 209 primer pairs were designed for all SSR sites.Conclusion This work extend the transcriptome information of S.glabra,which will provide gene resources for further study the biosynthesis pathway of active ingredients in S.glabra and facilitate molecular breeding in this species.
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