Identifying key genes involved in lateral root development of Bupleurum falcatum based on transcriptome data
Objective To identify key genes involved in the auxin signal transduction pathway that may participate in the lateral root development of Bupleurum falcatum.Methods Transcriptome sequencing of root tissues at different developmental stages of B.falcatum was performed using the Illumina HiSeq2500 high-throughput sequencing platform to screen for differentially expressed auxin signaling genes.The effects of exogenous IAA and TIBA on the lateral root development and expression of differentially expressed genes in B.falcatum seedlings were examined.Results A total of 138 DEGs related to the auxin signal transduction pathway were identified in the transcriptome data of seedling-stage B.falcatum,with 73 genes specifically highly expressed in 15-day-old the main root(S3).Correlation analysis revealed that 31 DEGs,including BfIAA9/14/16,BfARF6/19,BfGH3.6,and BfLAX3,showed highly correlated expression patterns,suggesting their potential involvement in the lateral root development of B.falcatum.Exogenous hormone induction results showed that low concentrations of IAA and TIBA treatments significantly increased the number of lateral roots in B.falcatum and promoted the expression of genes such as BfIAA9/14/16 and BfARF19.These results confirmed the accuracy of the transcriptome screening,indicating that BfIAA9/14/16 and BfARF19 may be key positive regulators of lateral root development in B.falcatum.Conclusion This study reveals significant differential expression patterns of auxin signaling pathway genes in the roots of B.falcatum at different developmental stages and tissues,with genes highly expressed in S3 tissue potentially being closely related to lateral root development.
NETL
NSTL
万方数据
