Objective To investigate the chemical constituents of Carthamus tinctorius. Methods The compounds were isolated and purified by various column chromatography over microporous resin,silica gel,Sephadex LH-20,ODS,and semi-preparative HPLC. The structures were elucidated by physicochemical characteristics and spectroscopic data including UV,IR,HR-ESI-MS,NMR,as well as ECD calculations. All the compounds were evaluated for their protective effects against OGD/R-stimulated H9C2 cell injury. Results A total of four compounds were isolated from the 95％ EtOH extract of C. tinctorius and elucidated as (−)-(3Z,1R,5S,9S,11R)-3-en-caryophyll-8(13)-en-5-ol-14-O-β-D-glucopyranoside (1),(−)-(3Z,1R,5S,9S)-3-en-caryophyll-8(13)-en-5-O-β-D-glucopyranoside (2),myrtenyl-10-O-β-D-glucopyranoside (3),geranyl-1-O-β-D-glucopyranoside (4). Compounds 1 and 4 could significantly protect OGD/R-induced H9C2 cell injury,with increase of cell viabilities by (25.01±2.01)％ and (29.99±0.37)％ at the concentration of 25 μmol/L,respectively. Conclusion Compounds 1 and 2 were new sesquiterpene glucosides,named saffloterpenoside A (1) and saffloterpenoside B (2). Compounds 3 and 4 were isolated from the Carthamus plants for the first time. Compounds 1 and 4 exhibited protective effects on OGD/R-injured H9C2 cells.
Carthamus tinctorius L.sesquiterpene glucosidemonoterpene glucosidecardioprotective activitymyrtenyl-10-O-β-D-glucopyranosidegeranyl-1-O-β-D-glucopyranosidesaffloterpenoside Asaffloterpenoside B
NETL
NSTL
万方数据
