Two new sesquiterpenoid glucosides from Carthamus tinctorius
Objective To investigate the chemical constituents of Carthamus tinctorius. Methods The compounds were isolated and purified by various column chromatography over microporous resin,silica gel,Sephadex LH-20,ODS,and semi-preparative HPLC. The structures were elucidated by physicochemical characteristics and spectroscopic data including UV,IR,HR-ESI-MS,NMR,as well as ECD calculations. All the compounds were evaluated for their protective effects against OGD/R-stimulated H9C2 cell injury. Results A total of four compounds were isolated from the 95％ EtOH extract of C. tinctorius and elucidated as (−)-(3Z,1R,5S,9S,11R)-3-en-caryophyll-8(13)-en-5-ol-14-O-β-D-glucopyranoside (1),(−)-(3Z,1R,5S,9S)-3-en-caryophyll-8(13)-en-5-O-β-D-glucopyranoside (2),myrtenyl-10-O-β-D-glucopyranoside (3),geranyl-1-O-β-D-glucopyranoside (4). Compounds 1 and 4 could significantly protect OGD/R-induced H9C2 cell injury,with increase of cell viabilities by (25.01±2.01)％ and (29.99±0.37)％ at the concentration of 25 μmol/L,respectively. Conclusion Compounds 1 and 2 were new sesquiterpene glucosides,named saffloterpenoside A (1) and saffloterpenoside B (2). Compounds 3 and 4 were isolated from the Carthamus plants for the first time. Compounds 1 and 4 exhibited protective effects on OGD/R-injured H9C2 cells.
Carthamus tinctorius L.sesquiterpene glucosidemonoterpene glucosidecardioprotective activitymyrtenyl-10-O-β-D-glucopyranosidegeranyl-1-O-β-D-glucopyranosidesaffloterpenoside Asaffloterpenoside B
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