Chemical constituents of Piper sarmentosum and their anti-neuroinflammatory activity
Objective This study aims to investigate the chemical constituents of Piper sarmentosum and their anti-neuroinflammatory activities. Methods A variety of column chromatographic techniques were used to separate and purify,and the structure of the compounds was identified by spectral and physiochemical data,and the anti-neuroinflammatory activity of these compounds was tested. Results A total of 18 compounds were isolated from 95％ ethanol extract of P. sarmentosum and were identified as (Z)-3-(3,4-dimethoxyphenyl)-1-(pyrrolidin-1-yl)prop-2-en-1-one (1),aurantiamide acetate (2),sarmentamide B (3),N-p-coumaroyltyramine (4),piperlotine D (5),piperlotine C (6),pellitorine (7),sarmentine (8),piperlotine J (9),cepharanone B (10),piperolactam B (11),tatarinoid C (12),1-(2,4,5-trimethoxyphenyl)-1,2-propanedione (13),3-hydroxy-β-damascone (14),(3E)-4-[(3R,4S)-3,4-dihydroxy-2,6,6-trimethyl-1-cyclohexen-1-yl]-3-buten-2-one (15),vomifoliol (16),6,7-dehydro-7,8-dihydro-3-oxo-α-ionol (17),3-oxo-α-ionol (18). Compound 2 had inhibitory effect on NO release of LPS-stimulated BV2 cells with IC50 value of (10.77±3.19) μmol/L. Compounds 4 and 8 inhibited acetylcholinesterase with IC50 values of (48.82±3.40) and (45.62±5.32) μmol/L,respectively. Conclusion Compound 1 was a new amide alkaloid and named as piperine B. Compounds 11 and 12 were isolated from P. sarmentosum for the first time. Compound 2 showed significantly inhibited NO release activity,and compounds 4 and 8 had certain acetylcholinesterase inhibitory activity.
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